Hypoosmotic swelling test in epididymal ram (Ovis aries) spermatozoa

Descripción del Articulo

This research was supported in part by the Peruvian National Council of Sciences, Technology and Innovation (CONCYTEC) through the Grant PROCYT315-2009 .
Detalles Bibliográficos
Autores: Vásquez J., Florentini E.A., Camargo L.A., Gonzales J., Valdivia M.
Formato: artículo
Fecha de Publicación:2013
Institución:Consejo Nacional de Ciencia Tecnología e Innovación
Repositorio:CONCYTEC-Institucional
Lenguaje:inglés
OAI Identifier:oai:repositorio.concytec.gob.pe:20.500.12390/932
Enlace del recurso:https://hdl.handle.net/20.500.12390/932
https://doi.org/10.1016/j.livsci.2013.08.023
Nivel de acceso:acceso abierto
Materia:Zoología
Genética animal
https://purl.org/pe-repo/ocde/ford#1.06.11
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oai_identifier_str oai:repositorio.concytec.gob.pe:20.500.12390/932
network_acronym_str CONC
network_name_str CONCYTEC-Institucional
repository_id_str 4689
dc.title.none.fl_str_mv Hypoosmotic swelling test in epididymal ram (Ovis aries) spermatozoa
title Hypoosmotic swelling test in epididymal ram (Ovis aries) spermatozoa
spellingShingle Hypoosmotic swelling test in epididymal ram (Ovis aries) spermatozoa
Vásquez J.
Zoología
Genética animal
https://purl.org/pe-repo/ocde/ford#1.06.11
title_short Hypoosmotic swelling test in epididymal ram (Ovis aries) spermatozoa
title_full Hypoosmotic swelling test in epididymal ram (Ovis aries) spermatozoa
title_fullStr Hypoosmotic swelling test in epididymal ram (Ovis aries) spermatozoa
title_full_unstemmed Hypoosmotic swelling test in epididymal ram (Ovis aries) spermatozoa
title_sort Hypoosmotic swelling test in epididymal ram (Ovis aries) spermatozoa
author Vásquez J.
author_facet Vásquez J.
Florentini E.A.
Camargo L.A.
Gonzales J.
Valdivia M.
author_role author
author2 Florentini E.A.
Camargo L.A.
Gonzales J.
Valdivia M.
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Vásquez J.
Florentini E.A.
Camargo L.A.
Gonzales J.
Valdivia M.
dc.subject.none.fl_str_mv Zoología
topic Zoología
Genética animal
https://purl.org/pe-repo/ocde/ford#1.06.11
dc.subject.es_PE.fl_str_mv Genética animal
dc.subject.ocde.none.fl_str_mv https://purl.org/pe-repo/ocde/ford#1.06.11
description This research was supported in part by the Peruvian National Council of Sciences, Technology and Innovation (CONCYTEC) through the Grant PROCYT315-2009 .
publishDate 2013
dc.date.accessioned.none.fl_str_mv 2024-05-30T23:13:38Z
dc.date.available.none.fl_str_mv 2024-05-30T23:13:38Z
dc.date.issued.fl_str_mv 2013
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.uri.none.fl_str_mv https://hdl.handle.net/20.500.12390/932
dc.identifier.doi.none.fl_str_mv https://doi.org/10.1016/j.livsci.2013.08.023
dc.identifier.scopus.none.fl_str_mv 2-s2.0-84886727969
url https://hdl.handle.net/20.500.12390/932
https://doi.org/10.1016/j.livsci.2013.08.023
identifier_str_mv 2-s2.0-84886727969
dc.language.iso.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONCYTEC-Institucional
instname:Consejo Nacional de Ciencia Tecnología e Innovación
instacron:CONCYTEC
instname_str Consejo Nacional de Ciencia Tecnología e Innovación
instacron_str CONCYTEC
institution CONCYTEC
reponame_str CONCYTEC-Institucional
collection CONCYTEC-Institucional
repository.name.fl_str_mv Repositorio Institucional CONCYTEC
repository.mail.fl_str_mv repositorio@concytec.gob.pe
_version_ 1844883059025903616
spelling Publicationrp02466600rp02467600rp02464600rp01216500rp02465600Vásquez J.Florentini E.A.Camargo L.A.Gonzales J.Valdivia M.2024-05-30T23:13:38Z2024-05-30T23:13:38Z2013https://hdl.handle.net/20.500.12390/932https://doi.org/10.1016/j.livsci.2013.08.0232-s2.0-84886727969This research was supported in part by the Peruvian National Council of Sciences, Technology and Innovation (CONCYTEC) through the Grant PROCYT315-2009 .The present study intended to develop the hypoosmotic swelling (HOS) test and to determine an appropriate hypoosmotic solution (HS) for its use in epididymal spermatozoa from ram, thus allowing the evaluation of the membrane functional integrity. We also tested if a 15 min exposure time could generate the same results as longer incubation times (30, 45 and 60 min). Ten different hypoosmotic solutions (50, 75, 100, 125, 150, 175, 200, 250, 275, and 300 mOsm/kg) were prepared from sodium citrate and fructose diluted in bidistilled water and were employed at 37 °C. According to the percentage of reacted spermatozoa, 75–100 mOsm/kg solutions were the most sensitive (P<0.05, n=15). Also, the different exposure times did not show any significant effect (P>0.05, n=15); therefore allowing 15 min to be considered sufficient incubation time.Consejo Nacional de Ciencia, Tecnología e Innovación Tecnológica - ConcytecengElsevierinfo:eu-repo/semantics/openAccessZoologíaGenética animal-1https://purl.org/pe-repo/ocde/ford#1.06.11-1Hypoosmotic swelling test in epididymal ram (Ovis aries) spermatozoainfo:eu-repo/semantics/articlereponame:CONCYTEC-Institucionalinstname:Consejo Nacional de Ciencia Tecnología e Innovacióninstacron:CONCYTEC20.500.12390/932oai:repositorio.concytec.gob.pe:20.500.12390/9322024-05-30 15:59:55.244http://purl.org/coar/access_right/c_14cbinfo:eu-repo/semantics/closedAccessmetadata only accesshttps://repositorio.concytec.gob.peRepositorio Institucional CONCYTECrepositorio@concytec.gob.pe#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#<Publication xmlns="https://www.openaire.eu/cerif-profile/1.1/" id="1a386ee7-a50f-4adc-98eb-7aeb3e3ff8f3"> <Type xmlns="https://www.openaire.eu/cerif-profile/vocab/COAR_Publication_Types">http://purl.org/coar/resource_type/c_1843</Type> <Language>eng</Language> <Title>Hypoosmotic swelling test in epididymal ram (Ovis aries) spermatozoa</Title> <PublishedIn> <Publication> </Publication> </PublishedIn> <PublicationDate>2013</PublicationDate> <DOI>https://doi.org/10.1016/j.livsci.2013.08.023</DOI> <SCP-Number>2-s2.0-84886727969</SCP-Number> <Authors> <Author> <DisplayName>Vásquez J.</DisplayName> <Person id="rp02466" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Florentini E.A.</DisplayName> <Person id="rp02467" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Camargo L.A.</DisplayName> <Person id="rp02464" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Gonzales J.</DisplayName> <Person id="rp01216" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Valdivia M.</DisplayName> <Person id="rp02465" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> </Authors> <Editors> </Editors> <Publishers> <Publisher> <DisplayName>Elsevier</DisplayName> <OrgUnit /> </Publisher> </Publishers> <Keyword>Zoología</Keyword> <Keyword>Genética animal</Keyword> <Abstract>The present study intended to develop the hypoosmotic swelling (HOS) test and to determine an appropriate hypoosmotic solution (HS) for its use in epididymal spermatozoa from ram, thus allowing the evaluation of the membrane functional integrity. We also tested if a 15 min exposure time could generate the same results as longer incubation times (30, 45 and 60 min). Ten different hypoosmotic solutions (50, 75, 100, 125, 150, 175, 200, 250, 275, and 300 mOsm/kg) were prepared from sodium citrate and fructose diluted in bidistilled water and were employed at 37 °C. According to the percentage of reacted spermatozoa, 75–100 mOsm/kg solutions were the most sensitive (P&lt;0.05, n=15). Also, the different exposure times did not show any significant effect (P&gt;0.05, n=15); therefore allowing 15 min to be considered sufficient incubation time.</Abstract> <Access xmlns="http://purl.org/coar/access_right" > </Access> </Publication> -1
score 13.36089
Hypoosmotic swelling test in epididymal ram (Ovis aries) spermatozoa
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La información contenida en este registro es de entera responsabilidad de la institución que gestiona el repositorio institucional donde esta contenido este documento o set de datos. El CONCYTEC no se hace responsable por los contenidos (publicaciones y/o datos) accesibles a través del Repositorio Nacional Digital de Ciencia, Tecnología e Innovación de Acceso Abierto (ALICIA).

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