Emergence and spread of carbapenem-resistant Acinetobacter baumannii international clones II and III in Lima, Peru article

• Autores: Levy-Blitchtein S., Roca I., Plasencia-Rebata S., Vicente-Taboada W., Velásquez-Pomar J., Muñoz L., Moreno-Morales J., Pons M.J., Del Valle-Mendoza J., Vila J.
• Formato: artículo
• Fecha de Publicación: 2018
• Institución: Consejo Nacional de Ciencia Tecnología e Innovación
• Repositorio: CONCYTEC-Institucional
• Lenguaje: inglés
• OAI Identifier: oai:repositorio.concytec.gob.pe:20.500.12390/590
• Enlace del recurso: https://hdl.handle.net/20.500.12390/590; https://doi.org/10.1038/s41426-018-0127-9
• Nivel de acceso: acceso abierto
• Materia: tertiary care center; carbapenem; carbapenem derivative; Acinetobacter baumannii; Article; bacterium isolation; carbapenem-resistant Enterobacteriaceae; gene insertion sequence; human; nonhuman; Peru; priority journal; pulsed field gel electrophoresis; South and Central America; Acinetobacter infection; bacterial gene; beta-lactam resistance; https://purl.org/pe-repo/ocde/ford#1.06.011

Carbapenem-resistant Acinetobacter baumannii is the top-ranked pathogen in the World Health Organization priority list of antibiotic-resistant bacteria. It emerged as a global pathogen due to the successful expansion of a few epidemic lineages, or international clones (ICs), producing acquired class D carbapenemases (OXA-type). During the past decade, however, reports regarding IC-I isolates in Latin America are scarce and are non-existent for IC-II and IC-III isolates. This study evaluates the molecular mechanisms of carbapenem resistance and the epidemiology of 80 non-duplicate clinical samples of A. baumannii collected from February 2014 through April 2016 at two tertiary care hospitals in Lima. Almost all isolates were carbapenem-resistant (97.5%), and susceptibility only remained high for colistin (95%). Pulsed-field gel electrophoresis showed two main clusters spread between both hospitals: cluster D containing 51 isolates (63.8%) associated with sequence type 2 (ST2) and carrying OXA-72, and cluster F containing 13 isolates (16.3%) associated with ST79 and also carrying OXA-72. ST2 and ST79 were endemic in at least one of the hospitals. ST1 and ST3 OXA-23-producing isolates were also identified. They accounted for sporadic hospital isolates. Interestingly, two isolates carried the novel OXA-253 variant of OXA-143 together with an upstream novel insertion sequence (ISAba47). While the predominant A. baumannii lineages in Latin America are linked to ST79, ST25, ST15, and ST1 producing OXA-23 enzymes, we report the emergence of highly resistant ST2 (IC-II) isolates in Peru producing OXA-72 and the first identification of ST3 isolates (IC-III) in Latin America, both considered a serious threat to public health worldwide.