Neural lineage cells produced by diferentiation of adipose stem cells for regenerative therapy in canine [Células de linaje neural producidas por diferenciación de células madre adiposas para terapia regenerativa en caninos]

Descripción del Articulo

Este trabajo se realizó con el apoyo financiero del Consejo Nacional de Ciencia y Tecnología (CONCYTEC) mediante convenio No. 409-2012-CONCYTEC-OAJ, así como por el convenio No. 541-FIDECOM-INNOVATE PERÚ-PIMEN-2015.
Detalles Bibliográficos
Autores: Enciso-Benavides J., Amiel-Pérez J., Rojas-Morán N., Enciso-Gutiérrez J.
Formato: artículo
Fecha de Publicación:2018
Institución:Consejo Nacional de Ciencia Tecnología e Innovación
Repositorio:CONCYTEC-Institucional
Lenguaje:español
OAI Identifier:oai:repositorio.concytec.gob.pe:20.500.12390/940
Enlace del recurso:https://hdl.handle.net/20.500.12390/940
Nivel de acceso:acceso abierto
Materia:Diferenciación neural
Célula madre
Adiposa
https://purl.org/pe-repo/ocde/ford#1.06.11
id CONC_33a7371d141dc80477f359b0710f8d4a
oai_identifier_str oai:repositorio.concytec.gob.pe:20.500.12390/940
network_acronym_str CONC
network_name_str CONCYTEC-Institucional
repository_id_str 4689
dc.title.none.fl_str_mv Neural lineage cells produced by diferentiation of adipose stem cells for regenerative therapy in canine [Células de linaje neural producidas por diferenciación de células madre adiposas para terapia regenerativa en caninos]
title Neural lineage cells produced by diferentiation of adipose stem cells for regenerative therapy in canine [Células de linaje neural producidas por diferenciación de células madre adiposas para terapia regenerativa en caninos]
spellingShingle Neural lineage cells produced by diferentiation of adipose stem cells for regenerative therapy in canine [Células de linaje neural producidas por diferenciación de células madre adiposas para terapia regenerativa en caninos]
Enciso-Benavides J.
Diferenciación neural
Célula madre
Adiposa
https://purl.org/pe-repo/ocde/ford#1.06.11
title_short Neural lineage cells produced by diferentiation of adipose stem cells for regenerative therapy in canine [Células de linaje neural producidas por diferenciación de células madre adiposas para terapia regenerativa en caninos]
title_full Neural lineage cells produced by diferentiation of adipose stem cells for regenerative therapy in canine [Células de linaje neural producidas por diferenciación de células madre adiposas para terapia regenerativa en caninos]
title_fullStr Neural lineage cells produced by diferentiation of adipose stem cells for regenerative therapy in canine [Células de linaje neural producidas por diferenciación de células madre adiposas para terapia regenerativa en caninos]
title_full_unstemmed Neural lineage cells produced by diferentiation of adipose stem cells for regenerative therapy in canine [Células de linaje neural producidas por diferenciación de células madre adiposas para terapia regenerativa en caninos]
title_sort Neural lineage cells produced by diferentiation of adipose stem cells for regenerative therapy in canine [Células de linaje neural producidas por diferenciación de células madre adiposas para terapia regenerativa en caninos]
author Enciso-Benavides J.
author_facet Enciso-Benavides J.
Amiel-Pérez J.
Rojas-Morán N.
Enciso-Gutiérrez J.
author_role author
author2 Amiel-Pérez J.
Rojas-Morán N.
Enciso-Gutiérrez J.
author2_role author
author
author
dc.contributor.author.fl_str_mv Enciso-Benavides J.
Amiel-Pérez J.
Rojas-Morán N.
Enciso-Gutiérrez J.
dc.subject.none.fl_str_mv Diferenciación neural
topic Diferenciación neural
Célula madre
Adiposa
https://purl.org/pe-repo/ocde/ford#1.06.11
dc.subject.es_PE.fl_str_mv Célula madre
Adiposa
dc.subject.ocde.none.fl_str_mv https://purl.org/pe-repo/ocde/ford#1.06.11
description Este trabajo se realizó con el apoyo financiero del Consejo Nacional de Ciencia y Tecnología (CONCYTEC) mediante convenio No. 409-2012-CONCYTEC-OAJ, así como por el convenio No. 541-FIDECOM-INNOVATE PERÚ-PIMEN-2015.
publishDate 2018
dc.date.accessioned.none.fl_str_mv 2024-05-30T23:13:38Z
dc.date.available.none.fl_str_mv 2024-05-30T23:13:38Z
dc.date.issued.fl_str_mv 2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.uri.none.fl_str_mv https://hdl.handle.net/20.500.12390/940
dc.identifier.scopus.none.fl_str_mv 2-s2.0-85048475737
url https://hdl.handle.net/20.500.12390/940
identifier_str_mv 2-s2.0-85048475737
dc.language.iso.none.fl_str_mv spa
language spa
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Universidad de Zulia
publisher.none.fl_str_mv Universidad de Zulia
dc.source.none.fl_str_mv reponame:CONCYTEC-Institucional
instname:Consejo Nacional de Ciencia Tecnología e Innovación
instacron:CONCYTEC
instname_str Consejo Nacional de Ciencia Tecnología e Innovación
instacron_str CONCYTEC
institution CONCYTEC
reponame_str CONCYTEC-Institucional
collection CONCYTEC-Institucional
repository.name.fl_str_mv Repositorio Institucional CONCYTEC
repository.mail.fl_str_mv repositorio@concytec.gob.pe
_version_ 1844883027538214912
spelling Publicationrp02493600rp02494600rp02492600rp02495600Enciso-Benavides J.Amiel-Pérez J.Rojas-Morán N.Enciso-Gutiérrez J.2024-05-30T23:13:38Z2024-05-30T23:13:38Z2018https://hdl.handle.net/20.500.12390/9402-s2.0-85048475737Este trabajo se realizó con el apoyo financiero del Consejo Nacional de Ciencia y Tecnología (CONCYTEC) mediante convenio No. 409-2012-CONCYTEC-OAJ, así como por el convenio No. 541-FIDECOM-INNOVATE PERÚ-PIMEN-2015.Stem cells facilitate nerve regeneration by producing neurotrophic factors and differentiating into any type of neural lineage. Acute and chronic pathologies of the nervous system can compromise one or more of these lineages, so regenerative therapy with allogeneic stem cells will give better results if they are predifferentiated. The objective of this work was to demonstrate that the canine adipose derived mesenchymal stem cell (cAd-MSC) isolates were transdifferentiated into neural lineage in a high percentage population, using a protocol of pre induction and induction and in a determined time. Consequently, 5-10 grames (g) of five dog fat was treated at 37o C with Type I collagenase, the pellet obtained was seeded in Dulbecco’s Modified Eagle’s Medium + 10 % Fetal Bovine Serum + penicillin / streptomycin/ amphotericin B. Cells presented in passages 2-5, cASC were characterized by evaluating their ability to differentiate in threelineages, assess their pluripotency identifying the transcription factor Oct4 expresion and evaluating the morphology by scanning electron microscopy. Differentiation to neural lineage was performed in two treatment steps: pre-induction using basicfibroblast growth factor (bFGF) and induction using 2% Dimethyl sulfoxide and 200 mM of butylated hydroxyanisole (BHA).Identification of the neural lineage (nestin+) was performed by immunohistochemistry at different hours (h) after induction, counting 400 cells. The results show that the isolates were adherent to plastic and fibroblast morphology, multipotent whendifferentiated into osteocytes, chondrocytes and adipocytes, most of them were Oct4 + (55.4% ± 1.86); induced cells were able to differentiate into nestin+ cells (70%), with neuronal and neuronalmorphology four h ost induction. It was concluded that 70% of adipose derived mesenchymal stem cells present in passages 2-5 differentiated into nestin positive cells with neural and neural morphology four h post induction, and could be used in fresh orcryopreserved cell therapy. This is the first report of differentiated canine adipose stem cell culture to neural lineage in Peru.Consejo Nacional de Ciencia, Tecnología e Innovación Tecnológica - ConcytecspaUniversidad de Zuliainfo:eu-repo/semantics/openAccessDiferenciación neuralCélula madre-1Adiposa-1https://purl.org/pe-repo/ocde/ford#1.06.11-1Neural lineage cells produced by diferentiation of adipose stem cells for regenerative therapy in canine [Células de linaje neural producidas por diferenciación de células madre adiposas para terapia regenerativa en caninos]info:eu-repo/semantics/articlereponame:CONCYTEC-Institucionalinstname:Consejo Nacional de Ciencia Tecnología e Innovacióninstacron:CONCYTEC20.500.12390/940oai:repositorio.concytec.gob.pe:20.500.12390/9402024-05-30 16:00:02.013http://purl.org/coar/access_right/c_14cbinfo:eu-repo/semantics/closedAccessmetadata only accesshttps://repositorio.concytec.gob.peRepositorio Institucional CONCYTECrepositorio@concytec.gob.pe#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#<Publication xmlns="https://www.openaire.eu/cerif-profile/1.1/" id="c46abcc8-fb3c-4227-b449-077b5df6d966"> <Type xmlns="https://www.openaire.eu/cerif-profile/vocab/COAR_Publication_Types">http://purl.org/coar/resource_type/c_1843</Type> <Language>spa</Language> <Title>Neural lineage cells produced by diferentiation of adipose stem cells for regenerative therapy in canine [Células de linaje neural producidas por diferenciación de células madre adiposas para terapia regenerativa en caninos]</Title> <PublishedIn> <Publication> </Publication> </PublishedIn> <PublicationDate>2018</PublicationDate> <SCP-Number>2-s2.0-85048475737</SCP-Number> <Authors> <Author> <DisplayName>Enciso-Benavides J.</DisplayName> <Person id="rp02493" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Amiel-Pérez J.</DisplayName> <Person id="rp02494" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Rojas-Morán N.</DisplayName> <Person id="rp02492" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Enciso-Gutiérrez J.</DisplayName> <Person id="rp02495" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> </Authors> <Editors> </Editors> <Publishers> <Publisher> <DisplayName>Universidad de Zulia</DisplayName> <OrgUnit /> </Publisher> </Publishers> <Keyword>Diferenciación neural</Keyword> <Keyword>Célula madre</Keyword> <Keyword>Adiposa</Keyword> <Abstract>Stem cells facilitate nerve regeneration by producing neurotrophic factors and differentiating into any type of neural lineage. Acute and chronic pathologies of the nervous system can compromise one or more of these lineages, so regenerative therapy with allogeneic stem cells will give better results if they are predifferentiated. The objective of this work was to demonstrate that the canine adipose derived mesenchymal stem cell (cAd-MSC) isolates were transdifferentiated into neural lineage in a high percentage population, using a protocol of pre induction and induction and in a determined time. Consequently, 5-10 grames (g) of five dog fat was treated at 37o C with Type I collagenase, the pellet obtained was seeded in Dulbecco’s Modified Eagle’s Medium + 10 % Fetal Bovine Serum + penicillin / streptomycin/ amphotericin B. Cells presented in passages 2-5, cASC were characterized by evaluating their ability to differentiate in threelineages, assess their pluripotency identifying the transcription factor Oct4 expresion and evaluating the morphology by scanning electron microscopy. Differentiation to neural lineage was performed in two treatment steps: pre-induction using basicfibroblast growth factor (bFGF) and induction using 2% Dimethyl sulfoxide and 200 mM of butylated hydroxyanisole (BHA).Identification of the neural lineage (nestin+) was performed by immunohistochemistry at different hours (h) after induction, counting 400 cells. The results show that the isolates were adherent to plastic and fibroblast morphology, multipotent whendifferentiated into osteocytes, chondrocytes and adipocytes, most of them were Oct4 + (55.4% ± 1.86); induced cells were able to differentiate into nestin+ cells (70%), with neuronal and neuronalmorphology four h ost induction. It was concluded that 70% of adipose derived mesenchymal stem cells present in passages 2-5 differentiated into nestin positive cells with neural and neural morphology four h post induction, and could be used in fresh orcryopreserved cell therapy. This is the first report of differentiated canine adipose stem cell culture to neural lineage in Peru.</Abstract> <Access xmlns="http://purl.org/coar/access_right" > </Access> </Publication> -1
score 13.361153
Neural lineage cells produced by diferentiation of adipose stem cells for regenerative therapy in canine [Células de linaje neural producidas por diferenciación de células madre adiposas para terapia regenerativa en caninos]
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