Preliminary protocol development of a hplc-tbars-evsc (Ex vivo stratum corneum) assay for skin research: Application in a sunscreen system
Descripción del Articulo
This research was funded by Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP), grant numbers 2016/24360-4 and 2019/16169-0; Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), process 305250/2019-1; CONCYTEC-FONDECYT, process E041-01 (Contract number 123-2018-FONDECYT-B...
| Autores: | , , , , , |
|---|---|
| Formato: | artículo |
| Fecha de Publicación: | 2021 |
| Institución: | Consejo Nacional de Ciencia Tecnología e Innovación |
| Repositorio: | CONCYTEC-Institucional |
| Lenguaje: | inglés |
| OAI Identifier: | oai:repositorio.concytec.gob.pe:20.500.12390/3045 |
| Enlace del recurso: | https://hdl.handle.net/20.500.12390/3045 https://doi.org/10.3390/scipharm89020017 |
| Nivel de acceso: | acceso abierto |
| Materia: | TBARS HPLC Stratum corneum oxidative stress Tape stripping https://purl.org/pe-repo/ocde/ford#3.02.27 |
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Preliminary protocol development of a hplc-tbars-evsc (Ex vivo stratum corneum) assay for skin research: Application in a sunscreen system |
| title |
Preliminary protocol development of a hplc-tbars-evsc (Ex vivo stratum corneum) assay for skin research: Application in a sunscreen system |
| spellingShingle |
Preliminary protocol development of a hplc-tbars-evsc (Ex vivo stratum corneum) assay for skin research: Application in a sunscreen system Sauce, Rafael TBARS HPLC Stratum corneum oxidative stress Stratum corneum oxidative stress Tape stripping https://purl.org/pe-repo/ocde/ford#3.02.27 |
| title_short |
Preliminary protocol development of a hplc-tbars-evsc (Ex vivo stratum corneum) assay for skin research: Application in a sunscreen system |
| title_full |
Preliminary protocol development of a hplc-tbars-evsc (Ex vivo stratum corneum) assay for skin research: Application in a sunscreen system |
| title_fullStr |
Preliminary protocol development of a hplc-tbars-evsc (Ex vivo stratum corneum) assay for skin research: Application in a sunscreen system |
| title_full_unstemmed |
Preliminary protocol development of a hplc-tbars-evsc (Ex vivo stratum corneum) assay for skin research: Application in a sunscreen system |
| title_sort |
Preliminary protocol development of a hplc-tbars-evsc (Ex vivo stratum corneum) assay for skin research: Application in a sunscreen system |
| author |
Sauce, Rafael |
| author_facet |
Sauce, Rafael Pinto, Claudineia Aparecida Sales de Oliveira Ayala-Jara, Carmen Prieto Lara, Zulita Adriana Robles Velasco, Maria Valéria Rolim Baby, André |
| author_role |
author |
| author2 |
Pinto, Claudineia Aparecida Sales de Oliveira Ayala-Jara, Carmen Prieto Lara, Zulita Adriana Robles Velasco, Maria Valéria Rolim Baby, André |
| author2_role |
author author author author author |
| dc.contributor.author.fl_str_mv |
Sauce, Rafael Pinto, Claudineia Aparecida Sales de Oliveira Ayala-Jara, Carmen Prieto Lara, Zulita Adriana Robles Velasco, Maria Valéria Rolim Baby, André |
| dc.subject.none.fl_str_mv |
TBARS |
| topic |
TBARS HPLC Stratum corneum oxidative stress Stratum corneum oxidative stress Tape stripping https://purl.org/pe-repo/ocde/ford#3.02.27 |
| dc.subject.es_PE.fl_str_mv |
HPLC Stratum corneum oxidative stress Stratum corneum oxidative stress Tape stripping |
| dc.subject.ocde.none.fl_str_mv |
https://purl.org/pe-repo/ocde/ford#3.02.27 |
| description |
This research was funded by Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP), grant numbers 2016/24360-4 and 2019/16169-0; Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), process 305250/2019-1; CONCYTEC-FONDECYT, process E041-01 (Contract number 123-2018-FONDECYT-BM-IADT/AV); and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior-Brasil (CAPES) Finance Code 001. |
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2021 |
| dc.date.accessioned.none.fl_str_mv |
2024-05-30T23:13:38Z |
| dc.date.available.none.fl_str_mv |
2024-05-30T23:13:38Z |
| dc.date.issued.fl_str_mv |
2021 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.uri.none.fl_str_mv |
https://hdl.handle.net/20.500.12390/3045 |
| dc.identifier.doi.none.fl_str_mv |
https://doi.org/10.3390/scipharm89020017 |
| dc.identifier.scopus.none.fl_str_mv |
2-s2.0-85106583344 |
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https://hdl.handle.net/20.500.12390/3045 https://doi.org/10.3390/scipharm89020017 |
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2-s2.0-85106583344 |
| dc.language.iso.none.fl_str_mv |
eng |
| language |
eng |
| dc.relation.ispartof.none.fl_str_mv |
Scientia Pharmaceutica |
| dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess |
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https://creativecommons.org/licenses/by-nc-nd/4.0/ |
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openAccess |
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https://creativecommons.org/licenses/by-nc-nd/4.0/ |
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MDPI |
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MDPI |
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reponame:CONCYTEC-Institucional instname:Consejo Nacional de Ciencia Tecnología e Innovación instacron:CONCYTEC |
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Consejo Nacional de Ciencia Tecnología e Innovación |
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CONCYTEC |
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CONCYTEC-Institucional |
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Publicationrp08715600rp08716600rp08717600rp08720600rp08718600rp08719600Sauce, RafaelPinto, Claudineia Aparecida Sales de OliveiraAyala-Jara, CarmenPrieto Lara, Zulita AdrianaRobles Velasco, Maria ValériaRolim Baby, André2024-05-30T23:13:38Z2024-05-30T23:13:38Z2021https://hdl.handle.net/20.500.12390/3045https://doi.org/10.3390/scipharm890200172-s2.0-85106583344This research was funded by Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP), grant numbers 2016/24360-4 and 2019/16169-0; Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), process 305250/2019-1; CONCYTEC-FONDECYT, process E041-01 (Contract number 123-2018-FONDECYT-BM-IADT/AV); and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior-Brasil (CAPES) Finance Code 001.Considering the importance of the cutaneous tissue investigation and the need for the development of new protocols to non-invasively establish the safety and efficacy of dermocosmetics and topical products, we aimed at developing an HPLC-TBARS-EVSC (high performance liquid chromatography–thiobarbituric acid reactive species–ex vivo stratum corneum) assay for the lipid peroxidation measurement on subjects’ stratum corneum (SC) obtained by tape stripping; additionally, we applied the HPLC-TBARS-EVSC assay in an emulsified sunscreen system containing ethylhexyl triazone and bemotrizinol as UV filters. HPLC analysis was performed in isocratic mode with 35% methanol/65% phosphate buffer (pH 7.0) as the mobile phase. The diode detector was set at 532 nm to quantify the malondialdehyde (MDA)-TBA adduct. An ex vivo tape stripping method was applied in 10 volunteers in three pre-defined regions of the volar forearms: the control; the irradiated; and the site containing the sunscreen (2.0 mg·cm−2). Ten adhesive tapes per region were used for SC removal. An exclusive ex vivo protocol to measure SC lipid peroxidation was preliminarily developed with linearity and selectivity. The protocol suggested the use of an artificial irradiation dose (5506 KJ·m−2) to improve the assay response from the SC. The sunscreen system had a significative decrease in SC lipoperoxidative damage compared to the control. Our protocol can aid in the efficacy establishment of anti-UV and antioxidant agents, for instance, in studies that aim at elucidating the level of SC lipid peroxidation and even in carrying out baseline investigations characterizing different ethnicities and genders.Consejo Nacional de Ciencia, Tecnología e Innovación Tecnológica - ConcytecengMDPIScientia Pharmaceuticainfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/4.0/TBARSHPLC-1Stratum corneum oxidative stress-1Stratum corneum oxidative stress-1Tape stripping-1https://purl.org/pe-repo/ocde/ford#3.02.27-1Preliminary protocol development of a hplc-tbars-evsc (Ex vivo stratum corneum) assay for skin research: Application in a sunscreen systeminfo:eu-repo/semantics/articlereponame:CONCYTEC-Institucionalinstname:Consejo Nacional de Ciencia Tecnología e Innovacióninstacron:CONCYTECTHUMBNAIL2021_Preliminary Protocol Development of a HPLC-TBARS_EVSC- scipharm-89-00017.pdf.jpg2021_Preliminary Protocol Development of a HPLC-TBARS_EVSC- scipharm-89-00017.pdf.jpgIM Thumbnailimage/jpeg13545https://repositorio.concytec.gob.pe/bitstreams/a7a71036-39d6-8eb2-cf66-416bc71a367e/download2ff948afb3640ce88d6b1d99eb23e30fMD52ORIGINAL2021_Preliminary Protocol Development of a HPLC-TBARS_EVSC- scipharm-89-00017.pdf2021_Preliminary Protocol Development of a HPLC-TBARS_EVSC- scipharm-89-00017.pdfapplication/pdf922057https://repositorio.concytec.gob.pe/bitstreams/52009754-65d0-e064-850f-efbf25dd52d0/download65c3decfab572380374f104fb60c7fa8MD51TEXT2021_Preliminary Protocol Development of a HPLC-TBARS_EVSC- scipharm-89-00017.pdf.txt2021_Preliminary Protocol Development of a HPLC-TBARS_EVSC- scipharm-89-00017.pdf.txtExtracted texttext/plain36441https://repositorio.concytec.gob.pe/bitstreams/936b0fb5-ba17-455c-b0dc-7e9b03811ff1/downloadc7c05c40a83e7cf3fc1d07840d6987ccMD5320.500.12390/3045oai:repositorio.concytec.gob.pe:20.500.12390/30452024-06-19 21:04:41.63https://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessopen accesshttps://repositorio.concytec.gob.peRepositorio Institucional CONCYTECrepositorio@concytec.gob.pe#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#<Publication xmlns="https://www.openaire.eu/cerif-profile/1.1/" id="970ce03d-1b32-4f26-89d0-6bff003362ed"> <Type xmlns="https://www.openaire.eu/cerif-profile/vocab/COAR_Publication_Types">http://purl.org/coar/resource_type/c_1843</Type> <Language>eng</Language> <Title>Preliminary protocol development of a hplc-tbars-evsc (Ex vivo stratum corneum) assay for skin research: Application in a sunscreen system</Title> <PublishedIn> <Publication> <Title>Scientia Pharmaceutica</Title> </Publication> </PublishedIn> <PublicationDate>2021</PublicationDate> <DOI>https://doi.org/10.3390/scipharm89020017</DOI> <SCP-Number>2-s2.0-85106583344</SCP-Number> <Authors> <Author> <DisplayName>Sauce, Rafael</DisplayName> <Person id="rp08715" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Pinto, Claudineia Aparecida Sales de Oliveira</DisplayName> <Person id="rp08716" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Ayala-Jara, Carmen</DisplayName> <Person id="rp08717" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Prieto Lara, Zulita Adriana</DisplayName> <Person id="rp08720" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Robles Velasco, Maria Valéria</DisplayName> <Person id="rp08718" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Rolim Baby, André</DisplayName> <Person id="rp08719" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> </Authors> <Editors> </Editors> <Publishers> <Publisher> <DisplayName>MDPI</DisplayName> <OrgUnit /> </Publisher> </Publishers> <License>https://creativecommons.org/licenses/by-nc-nd/4.0/</License> <Keyword>TBARS</Keyword> <Keyword>HPLC</Keyword> <Keyword>Stratum corneum oxidative stress</Keyword> <Keyword>Stratum corneum oxidative stress</Keyword> <Keyword>Tape stripping</Keyword> <Abstract>Considering the importance of the cutaneous tissue investigation and the need for the development of new protocols to non-invasively establish the safety and efficacy of dermocosmetics and topical products, we aimed at developing an HPLC-TBARS-EVSC (high performance liquid chromatography–thiobarbituric acid reactive species–ex vivo stratum corneum) assay for the lipid peroxidation measurement on subjects’ stratum corneum (SC) obtained by tape stripping; additionally, we applied the HPLC-TBARS-EVSC assay in an emulsified sunscreen system containing ethylhexyl triazone and bemotrizinol as UV filters. HPLC analysis was performed in isocratic mode with 35% methanol/65% phosphate buffer (pH 7.0) as the mobile phase. The diode detector was set at 532 nm to quantify the malondialdehyde (MDA)-TBA adduct. An ex vivo tape stripping method was applied in 10 volunteers in three pre-defined regions of the volar forearms: the control; the irradiated; and the site containing the sunscreen (2.0 mg·cm−2). Ten adhesive tapes per region were used for SC removal. An exclusive ex vivo protocol to measure SC lipid peroxidation was preliminarily developed with linearity and selectivity. The protocol suggested the use of an artificial irradiation dose (5506 KJ·m−2) to improve the assay response from the SC. The sunscreen system had a significative decrease in SC lipoperoxidative damage compared to the control. Our protocol can aid in the efficacy establishment of anti-UV and antioxidant agents, for instance, in studies that aim at elucidating the level of SC lipid peroxidation and even in carrying out baseline investigations characterizing different ethnicities and genders.</Abstract> <Access xmlns="http://purl.org/coar/access_right" > </Access> </Publication> -1 |
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La información contenida en este registro es de entera responsabilidad de la institución que gestiona el repositorio institucional donde esta contenido este documento o set de datos. El CONCYTEC no se hace responsable por los contenidos (publicaciones y/o datos) accesibles a través del Repositorio Nacional Digital de Ciencia, Tecnología e Innovación de Acceso Abierto (ALICIA).
La información contenida en este registro es de entera responsabilidad de la institución que gestiona el repositorio institucional donde esta contenido este documento o set de datos. El CONCYTEC no se hace responsable por los contenidos (publicaciones y/o datos) accesibles a través del Repositorio Nacional Digital de Ciencia, Tecnología e Innovación de Acceso Abierto (ALICIA).
