Antioxidant properties of mashua (Tropaeolum tuberosum) phenolic extracts against oxidative damage using biological in vitro assays
Descripción del Articulo
We thank Dr. Carlos Arbizu, International Potato Center (Lima, Peru) for providing the mashua genotypes and Alexandrine During and Yves-Jacques Schneider for their suggestions in the discussion of this paper. This research was supported by the CUI project of the Belgian Coopération Universitaire au...
| Autores: | , , , , , |
|---|---|
| Formato: | artículo |
| Fecha de Publicación: | 2008 |
| Institución: | Consejo Nacional de Ciencia Tecnología e Innovación |
| Repositorio: | CONCYTEC-Institucional |
| Lenguaje: | inglés |
| OAI Identifier: | oai:repositorio.concytec.gob.pe:20.500.12390/722 |
| Enlace del recurso: | https://hdl.handle.net/20.500.12390/722 https://doi.org/10.1016/j.foodchem.2008.03.038 |
| Nivel de acceso: | acceso abierto |
| Materia: | polyunsaturated fatty acid alkadiene anthocyanin copper flavanoid gallic acid low density lipoprotein metal ion oxygen radical peroxy radical phenol derivative plant extract |
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CONC_0a1afc7e6bf18f1dcead9a8f5ab758f7 |
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oai:repositorio.concytec.gob.pe:20.500.12390/722 |
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CONC |
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CONCYTEC-Institucional |
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| dc.title.none.fl_str_mv |
Antioxidant properties of mashua (Tropaeolum tuberosum) phenolic extracts against oxidative damage using biological in vitro assays |
| title |
Antioxidant properties of mashua (Tropaeolum tuberosum) phenolic extracts against oxidative damage using biological in vitro assays |
| spellingShingle |
Antioxidant properties of mashua (Tropaeolum tuberosum) phenolic extracts against oxidative damage using biological in vitro assays Chirinos R. polyunsaturated fatty acid alkadiene anthocyanin copper flavanoid gallic acid low density lipoprotein metal ion oxygen radical peroxy radical phenol derivative plant extract |
| title_short |
Antioxidant properties of mashua (Tropaeolum tuberosum) phenolic extracts against oxidative damage using biological in vitro assays |
| title_full |
Antioxidant properties of mashua (Tropaeolum tuberosum) phenolic extracts against oxidative damage using biological in vitro assays |
| title_fullStr |
Antioxidant properties of mashua (Tropaeolum tuberosum) phenolic extracts against oxidative damage using biological in vitro assays |
| title_full_unstemmed |
Antioxidant properties of mashua (Tropaeolum tuberosum) phenolic extracts against oxidative damage using biological in vitro assays |
| title_sort |
Antioxidant properties of mashua (Tropaeolum tuberosum) phenolic extracts against oxidative damage using biological in vitro assays |
| author |
Chirinos R. |
| author_facet |
Chirinos R. Campos D. Warnier M. Pedreschi R. Rees J.-F. Larondelle Y. |
| author_role |
author |
| author2 |
Campos D. Warnier M. Pedreschi R. Rees J.-F. Larondelle Y. |
| author2_role |
author author author author author |
| dc.contributor.author.fl_str_mv |
Chirinos R. Campos D. Warnier M. Pedreschi R. Rees J.-F. Larondelle Y. |
| dc.subject.none.fl_str_mv |
polyunsaturated fatty acid |
| topic |
polyunsaturated fatty acid alkadiene anthocyanin copper flavanoid gallic acid low density lipoprotein metal ion oxygen radical peroxy radical phenol derivative plant extract |
| dc.subject.es_PE.fl_str_mv |
alkadiene anthocyanin copper flavanoid gallic acid low density lipoprotein metal ion oxygen radical peroxy radical phenol derivative plant extract |
| description |
We thank Dr. Carlos Arbizu, International Potato Center (Lima, Peru) for providing the mashua genotypes and Alexandrine During and Yves-Jacques Schneider for their suggestions in the discussion of this paper. This research was supported by the CUI project of the Belgian Coopération Universitaire au Développement (CUD, Belgium) and by the Consejo Nacional de Ciencia y Tecnología (CONCYTEC, Peru). |
| publishDate |
2008 |
| dc.date.accessioned.none.fl_str_mv |
2024-05-30T23:13:38Z |
| dc.date.available.none.fl_str_mv |
2024-05-30T23:13:38Z |
| dc.date.issued.fl_str_mv |
2008 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.uri.none.fl_str_mv |
https://hdl.handle.net/20.500.12390/722 |
| dc.identifier.doi.none.fl_str_mv |
https://doi.org/10.1016/j.foodchem.2008.03.038 |
| dc.identifier.scopus.none.fl_str_mv |
2-s2.0-43649107657 |
| url |
https://hdl.handle.net/20.500.12390/722 https://doi.org/10.1016/j.foodchem.2008.03.038 |
| identifier_str_mv |
2-s2.0-43649107657 |
| dc.language.iso.none.fl_str_mv |
eng |
| language |
eng |
| dc.relation.ispartof.none.fl_str_mv |
Food Chemistry |
| dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess |
| dc.rights.uri.none.fl_str_mv |
https://creativecommons.org/licenses/by/4.0/ |
| eu_rights_str_mv |
openAccess |
| rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0/ |
| dc.publisher.en.fl_str_mv |
Elsevier Ltd Elsevier Ltd |
| dc.source.none.fl_str_mv |
reponame:CONCYTEC-Institucional instname:Consejo Nacional de Ciencia Tecnología e Innovación instacron:CONCYTEC |
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Consejo Nacional de Ciencia Tecnología e Innovación |
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CONCYTEC |
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CONCYTEC |
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CONCYTEC-Institucional |
| collection |
CONCYTEC-Institucional |
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Repositorio Institucional CONCYTEC |
| repository.mail.fl_str_mv |
repositorio@concytec.gob.pe |
| _version_ |
1844883110645202944 |
| spelling |
Publicationrp01538500rp01542500rp01804600rp01539500rp01805600rp01806600Chirinos R.Campos D.Warnier M.Pedreschi R.Rees J.-F.Larondelle Y.2024-05-30T23:13:38Z2024-05-30T23:13:38Z2008https://hdl.handle.net/20.500.12390/722https://doi.org/10.1016/j.foodchem.2008.03.0382-s2.0-43649107657We thank Dr. Carlos Arbizu, International Potato Center (Lima, Peru) for providing the mashua genotypes and Alexandrine During and Yves-Jacques Schneider for their suggestions in the discussion of this paper. This research was supported by the CUI project of the Belgian Coopération Universitaire au Développement (CUD, Belgium) and by the Consejo Nacional de Ciencia y Tecnología (CONCYTEC, Peru).Purified mashua extracts (PME) from four different coloured mashua genotypes were assayed for oxidative damage prevention. Three in vitro assays for oxidative damage to biological structures rich in polyunsaturated fatty acids (PUFA), such as LDL and erythrocytes, were tested: AAPH-induced TBARS assay and Cu2+-induced conjugated dienes assay for LDL oxidation and AAPH-induced oxidative hemolysis of erythrocytes. Additionally, ORAC antioxidant capacity, total phenolics (TP), total flavanoids (TFA) and total anthocyanins (TA) were evaluated. In the presence of 5 lM of gallic acid equivalents (GAE), inhibitions of LDL oxidation for the PME ranged from 29.1% to 34.8% and from 51.8% to 58.1% when the TBARS and conjugated dienes assays were performed, respectively. PME inhibited the hemolysis of erythrocytes within the range 20.8–25.1%. Thus, mashua phenolic extracts are capable of scavenging peroxyl radicals, as well as chelating redox metal ions in vitro. ORAC and LDL protection (TBARS and conjugated dienes assays) showed good correlations with the TP and TFA, suggesting that these compounds have a good ability to protect LDL molecules under the employed conditions. In contrast, inhibition of hemolysis did not show any correlation with the evaluated phenolic assays (TP, TA, TFA) or with any of the evaluated oxidative LDL assays, suggesting a specific action of some non-evaluated compounds present in the PME. The results of this study indicate that the mashua polyphenol extracts displayed good antioxidant properties against oxidative damage in biological structures rich in PUFA. The displayed antioxidant properties could be applied in the field of food or cosmetic industry.Purified mashua extracts (PME) from four different coloured mashua genotypes were assayed for oxidative damage prevention. Three in vitro assays for oxidative damage to biological structures rich in polyunsaturated fatty acids (PUFA), such as LDL and erythrocytes, were tested: AAPH-induced TBARS assay and Cu2+-induced conjugated dienes assay for LDL oxidation and AAPH-induced oxidative hemolysis of erythrocytes. Additionally, ORAC antioxidant capacity, total phenolics (TP), total flavanoids (TFA) and total anthocyanins (TA) were evaluated. In the presence of 5 lM of gallic acid equivalents (GAE), inhibitions of LDL oxidation for the PME ranged from 29.1% to 34.8% and from 51.8% to 58.1% when the TBARS and conjugated dienes assays were performed, respectively. PME inhibited the hemolysis of erythrocytes within the range 20.8–25.1%. Thus, mashua phenolic extracts are capable of scavenging peroxyl radicals, as well as chelating redox metal ions in vitro. ORAC and LDL protection (TBARS and conjugated dienes assays) showed good correlations with the TP and TFA, suggesting that these compounds have a good ability to protect LDL molecules under the employed conditions. In contrast, inhibition of hemolysis did not show any correlation with the evaluated phenolic assays (TP, TA, TFA) or with any of the evaluated oxidative LDL assays, suggesting a specific action of some non-evaluated compounds present in the PME. The results of this study indicate that the mashua polyphenol extracts displayed good antioxidant properties against oxidative damage in biological structures rich in PUFA. The displayed antioxidant properties could be applied in the field of food or cosmetic industry.Consejo Nacional de Ciencia, Tecnología e Innovación Tecnológica - ConcytecengElsevier LtdElsevier LtdFood Chemistryinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/4.0/polyunsaturated fatty acidalkadiene-1anthocyanin-1copper-1flavanoid-1gallic acid-1low density lipoprotein-1metal ion-1oxygen radical-1peroxy radical-1phenol derivative-1plant extract-1Antioxidant properties of mashua (Tropaeolum tuberosum) phenolic extracts against oxidative damage using biological in vitro assaysinfo:eu-repo/semantics/articlereponame:CONCYTEC-Institucionalinstname:Consejo Nacional de Ciencia Tecnología e Innovacióninstacron:CONCYTEC20.500.12390/722oai:repositorio.concytec.gob.pe:20.500.12390/7222025-09-23 12:02:09.965https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_14cbinfo:eu-repo/semantics/closedAccessmetadata only accesshttps://repositorio.concytec.gob.peRepositorio Institucional CONCYTECrepositorio@concytec.gob.pe#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#<Publication xmlns="https://www.openaire.eu/cerif-profile/1.1/" id="60a7c0a1-3a20-4fa7-9ee6-079cba79fac7"> <Type xmlns="https://www.openaire.eu/cerif-profile/vocab/COAR_Publication_Types">http://purl.org/coar/resource_type/c_1843</Type> <Language>eng</Language> <Title>Antioxidant properties of mashua (Tropaeolum tuberosum) phenolic extracts against oxidative damage using biological in vitro assays</Title> <PublishedIn> <Publication> <Title>Food Chemistry</Title> </Publication> </PublishedIn> <PublicationDate>2008</PublicationDate> <DOI>https://doi.org/10.1016/j.foodchem.2008.03.038</DOI> <SCP-Number>2-s2.0-43649107657</SCP-Number> <Authors> <Author> <DisplayName>Chirinos R.</DisplayName> <Person id="rp01538" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Campos D.</DisplayName> <Person id="rp01542" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Warnier M.</DisplayName> <Person id="rp01804" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Pedreschi R.</DisplayName> <Person id="rp01539" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Rees J.-F.</DisplayName> <Person id="rp01805" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Larondelle Y.</DisplayName> <Person id="rp01806" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> </Authors> <Editors> </Editors> <Publishers> <Publisher> <DisplayName>Elsevier Ltd</DisplayName> <DisplayName>Elsevier Ltd</DisplayName> <OrgUnit /> </Publisher> </Publishers> <License>https://creativecommons.org/licenses/by/4.0/</License> <Keyword>polyunsaturated fatty acid</Keyword> <Keyword>alkadiene</Keyword> <Keyword>anthocyanin</Keyword> <Keyword>copper</Keyword> <Keyword>flavanoid</Keyword> <Keyword>gallic acid</Keyword> <Keyword>low density lipoprotein</Keyword> <Keyword>metal ion</Keyword> <Keyword>oxygen radical</Keyword> <Keyword>peroxy radical</Keyword> <Keyword>phenol derivative</Keyword> <Keyword>plant extract</Keyword> <Abstract>Purified mashua extracts (PME) from four different coloured mashua genotypes were assayed for oxidative damage prevention. Three in vitro assays for oxidative damage to biological structures rich in polyunsaturated fatty acids (PUFA), such as LDL and erythrocytes, were tested: AAPH-induced TBARS assay and Cu2+-induced conjugated dienes assay for LDL oxidation and AAPH-induced oxidative hemolysis of erythrocytes. Additionally, ORAC antioxidant capacity, total phenolics (TP), total flavanoids (TFA) and total anthocyanins (TA) were evaluated. In the presence of 5 lM of gallic acid equivalents (GAE), inhibitions of LDL oxidation for the PME ranged from 29.1% to 34.8% and from 51.8% to 58.1% when the TBARS and conjugated dienes assays were performed, respectively. PME inhibited the hemolysis of erythrocytes within the range 20.8–25.1%. Thus, mashua phenolic extracts are capable of scavenging peroxyl radicals, as well as chelating redox metal ions in vitro. ORAC and LDL protection (TBARS and conjugated dienes assays) showed good correlations with the TP and TFA, suggesting that these compounds have a good ability to protect LDL molecules under the employed conditions. In contrast, inhibition of hemolysis did not show any correlation with the evaluated phenolic assays (TP, TA, TFA) or with any of the evaluated oxidative LDL assays, suggesting a specific action of some non-evaluated compounds present in the PME. The results of this study indicate that the mashua polyphenol extracts displayed good antioxidant properties against oxidative damage in biological structures rich in PUFA. The displayed antioxidant properties could be applied in the field of food or cosmetic industry.</Abstract> <Abstract>Purified mashua extracts (PME) from four different coloured mashua genotypes were assayed for oxidative damage prevention. Three in vitro assays for oxidative damage to biological structures rich in polyunsaturated fatty acids (PUFA), such as LDL and erythrocytes, were tested: AAPH-induced TBARS assay and Cu2+-induced conjugated dienes assay for LDL oxidation and AAPH-induced oxidative hemolysis of erythrocytes. Additionally, ORAC antioxidant capacity, total phenolics (TP), total flavanoids (TFA) and total anthocyanins (TA) were evaluated. In the presence of 5 lM of gallic acid equivalents (GAE), inhibitions of LDL oxidation for the PME ranged from 29.1% to 34.8% and from 51.8% to 58.1% when the TBARS and conjugated dienes assays were performed, respectively. PME inhibited the hemolysis of erythrocytes within the range 20.8–25.1%. Thus, mashua phenolic extracts are capable of scavenging peroxyl radicals, as well as chelating redox metal ions in vitro. ORAC and LDL protection (TBARS and conjugated dienes assays) showed good correlations with the TP and TFA, suggesting that these compounds have a good ability to protect LDL molecules under the employed conditions. In contrast, inhibition of hemolysis did not show any correlation with the evaluated phenolic assays (TP, TA, TFA) or with any of the evaluated oxidative LDL assays, suggesting a specific action of some non-evaluated compounds present in the PME. The results of this study indicate that the mashua polyphenol extracts displayed good antioxidant properties against oxidative damage in biological structures rich in PUFA. The displayed antioxidant properties could be applied in the field of food or cosmetic industry.</Abstract> <Access xmlns="http://purl.org/coar/access_right" > </Access> </Publication> -1 |
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13.813745 |
Nota importante:
La información contenida en este registro es de entera responsabilidad de la institución que gestiona el repositorio institucional donde esta contenido este documento o set de datos. El CONCYTEC no se hace responsable por los contenidos (publicaciones y/o datos) accesibles a través del Repositorio Nacional Digital de Ciencia, Tecnología e Innovación de Acceso Abierto (ALICIA).
La información contenida en este registro es de entera responsabilidad de la institución que gestiona el repositorio institucional donde esta contenido este documento o set de datos. El CONCYTEC no se hace responsable por los contenidos (publicaciones y/o datos) accesibles a través del Repositorio Nacional Digital de Ciencia, Tecnología e Innovación de Acceso Abierto (ALICIA).
