Characterization of native strains of Azotobacter spp. and its effect on growth of Lycopersicon esculentum Mill. “tomato” in Lambayeque

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The objective of this research was characterize and determine the effect of native strains of Azotobacter spp. on the vegetative growth of Lycopersicon esculentum Mill. "Tomato" as an alternative to the indiscriminate use of chemical fertilizers. Samples were taken from roots and rhizosphe...

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Detalles Bibliográficos
Autores: Escobar, Cynthia, Horna, Yuri, Carreño, Carmen, Mendoza, Gilmar
Formato: artículo
Fecha de Publicación:2011
Institución:Universidad Nacional de Trujillo
Repositorio:Revista UNITRU - Scientia Agropecuaria
Lenguaje:español
OAI Identifier:oai:ojs.revistas.unitru.edu.pe:article/37
Enlace del recurso:http://revistas.unitru.edu.pe/index.php/scientiaagrop/article/view/37
Nivel de acceso:acceso abierto
Materia:Azotobacter
PGPRs
Lycopersicon esculentum Mill.
indoleacetic acid.
ácido indolacético.
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spelling Characterization of native strains of Azotobacter spp. and its effect on growth of Lycopersicon esculentum Mill. “tomato” in LambayequeCaracterización de cepas nativas de Azotobacter spp. y su efecto en el desarrollo de Lycopersicon esculentum Mill. “tomate” en LambayequeEscobar, CynthiaHorna, YuriCarreño, CarmenMendoza, GilmarAzotobacterPGPRsLycopersicon esculentum Mill.indoleacetic acid.AzotobacterPGPRsLycopersicon esculentum Mill.ácido indolacético.The objective of this research was characterize and determine the effect of native strains of Azotobacter spp. on the vegetative growth of Lycopersicon esculentum Mill. "Tomato" as an alternative to the indiscriminate use of chemical fertilizers. Samples were taken from roots and rhizosphere soil of vegetables from which dilutions (10-4) in Ashby-sucrose broth and incubated at 30 °C to observe a yellow color, turbidity and surface film. The genus Azotobacter was identified in mineral agar without nitrogen and Ashby-benzoate, obtaining 96 strains with a yield of 7.10 to 57.99 mgL-1 indoleacetic acid, 0.13 to 1.64 mgL-1 of fixed nitrogen as ammonium and up to 1.61% efficiency in the solubilization of phosphate Bayovar rock. It obtained a cell suspension (108) of each of the four strains with the highest values and inoculated independently and in consortium, as well as a combination with 50% urea-100 % phosphate rock in the rhizosphere of Rio Grande tomato, in a completely randomized design. The entire native strains increased height, root volume, total dry matter, shoot and root, compared with absolute control.El objetivo de la presente investigación fue caracterizar y determinar el efecto de cepas nativas de Azotobacter spp. en el desarrollo vegetativo de Lycopersicon esculentum Mill. “tomate”, como una alternativa al uso indiscriminado de fertilizantes químicos. Se tomaron muestras de raíces y suelo rizosférico de hortalizas con las que se realizaron diluciones (10-4) en caldo Ashby-Sacarosa y se incubaron a 30 ºC hasta observar un color amarillo, turbidez y película superficial. El género Azotobacter se identificó en agar mineral sin nitrógeno y Ashby-Benzoato, obteniéndose 96 cepas con una producción de 7.10 a 57.99 mgL-1 de ácido indolacético, 0.13 a 1.64 mgL-1 de nitrógeno fijado como amonio y hasta 1.61 % de eficiencia en la solubilización de roca fosfórica de Bayóvar. Se obtuvo una suspensión celular (108) de cada una de las cuatro cepas con los mayores valores y se inocularon independientemente y en consorcio, así como una combinación con 50 % de urea-100 % de roca fosfórica, en la rizósfera de tomate cv. Río Grande, en un diseño experimental completamente aleatorio. Todas las cepas nativas incrementaron la altura, volumen radicular, materia seca total, parte aérea y radicular frente al testigo absoluto.Universidad Nacional de Trujillo2011-02-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://revistas.unitru.edu.pe/index.php/scientiaagrop/article/view/3710.17268/sci.agropecu.2011.01.05Scientia Agropecuaria; Vol. 2 No. 1 (2011): January - March; 39-49Scientia Agropecuaria; Vol. 2 Núm. 1 (2011): Enero - Marzo; 39-492306-67412077-9917reponame:Revista UNITRU - Scientia Agropecuariainstname:Universidad Nacional de Trujilloinstacron:UNITRUspahttp://revistas.unitru.edu.pe/index.php/scientiaagrop/article/view/37/53Derechos de autor 2011 Scientia Agropecuariainfo:eu-repo/semantics/openAccess2021-06-01T15:35:14Zmail@mail.com -
dc.title.none.fl_str_mv Characterization of native strains of Azotobacter spp. and its effect on growth of Lycopersicon esculentum Mill. “tomato” in Lambayeque
Caracterización de cepas nativas de Azotobacter spp. y su efecto en el desarrollo de Lycopersicon esculentum Mill. “tomate” en Lambayeque
title Characterization of native strains of Azotobacter spp. and its effect on growth of Lycopersicon esculentum Mill. “tomato” in Lambayeque
spellingShingle Characterization of native strains of Azotobacter spp. and its effect on growth of Lycopersicon esculentum Mill. “tomato” in Lambayeque
Escobar, Cynthia
Azotobacter
PGPRs
Lycopersicon esculentum Mill.
indoleacetic acid.
Azotobacter
PGPRs
Lycopersicon esculentum Mill.
ácido indolacético.
title_short Characterization of native strains of Azotobacter spp. and its effect on growth of Lycopersicon esculentum Mill. “tomato” in Lambayeque
title_full Characterization of native strains of Azotobacter spp. and its effect on growth of Lycopersicon esculentum Mill. “tomato” in Lambayeque
title_fullStr Characterization of native strains of Azotobacter spp. and its effect on growth of Lycopersicon esculentum Mill. “tomato” in Lambayeque
title_full_unstemmed Characterization of native strains of Azotobacter spp. and its effect on growth of Lycopersicon esculentum Mill. “tomato” in Lambayeque
title_sort Characterization of native strains of Azotobacter spp. and its effect on growth of Lycopersicon esculentum Mill. “tomato” in Lambayeque
dc.creator.none.fl_str_mv Escobar, Cynthia
Horna, Yuri
Carreño, Carmen
Mendoza, Gilmar
author Escobar, Cynthia
author_facet Escobar, Cynthia
Horna, Yuri
Carreño, Carmen
Mendoza, Gilmar
author_role author
author2 Horna, Yuri
Carreño, Carmen
Mendoza, Gilmar
author2_role author
author
author
dc.subject.none.fl_str_mv Azotobacter
PGPRs
Lycopersicon esculentum Mill.
indoleacetic acid.
Azotobacter
PGPRs
Lycopersicon esculentum Mill.
ácido indolacético.
topic Azotobacter
PGPRs
Lycopersicon esculentum Mill.
indoleacetic acid.
Azotobacter
PGPRs
Lycopersicon esculentum Mill.
ácido indolacético.
dc.description.none.fl_txt_mv The objective of this research was characterize and determine the effect of native strains of Azotobacter spp. on the vegetative growth of Lycopersicon esculentum Mill. "Tomato" as an alternative to the indiscriminate use of chemical fertilizers. Samples were taken from roots and rhizosphere soil of vegetables from which dilutions (10-4) in Ashby-sucrose broth and incubated at 30 °C to observe a yellow color, turbidity and surface film. The genus Azotobacter was identified in mineral agar without nitrogen and Ashby-benzoate, obtaining 96 strains with a yield of 7.10 to 57.99 mgL-1 indoleacetic acid, 0.13 to 1.64 mgL-1 of fixed nitrogen as ammonium and up to 1.61% efficiency in the solubilization of phosphate Bayovar rock. It obtained a cell suspension (108) of each of the four strains with the highest values and inoculated independently and in consortium, as well as a combination with 50% urea-100 % phosphate rock in the rhizosphere of Rio Grande tomato, in a completely randomized design. The entire native strains increased height, root volume, total dry matter, shoot and root, compared with absolute control.
El objetivo de la presente investigación fue caracterizar y determinar el efecto de cepas nativas de Azotobacter spp. en el desarrollo vegetativo de Lycopersicon esculentum Mill. “tomate”, como una alternativa al uso indiscriminado de fertilizantes químicos. Se tomaron muestras de raíces y suelo rizosférico de hortalizas con las que se realizaron diluciones (10-4) en caldo Ashby-Sacarosa y se incubaron a 30 ºC hasta observar un color amarillo, turbidez y película superficial. El género Azotobacter se identificó en agar mineral sin nitrógeno y Ashby-Benzoato, obteniéndose 96 cepas con una producción de 7.10 a 57.99 mgL-1 de ácido indolacético, 0.13 a 1.64 mgL-1 de nitrógeno fijado como amonio y hasta 1.61 % de eficiencia en la solubilización de roca fosfórica de Bayóvar. Se obtuvo una suspensión celular (108) de cada una de las cuatro cepas con los mayores valores y se inocularon independientemente y en consorcio, así como una combinación con 50 % de urea-100 % de roca fosfórica, en la rizósfera de tomate cv. Río Grande, en un diseño experimental completamente aleatorio. Todas las cepas nativas incrementaron la altura, volumen radicular, materia seca total, parte aérea y radicular frente al testigo absoluto.
description The objective of this research was characterize and determine the effect of native strains of Azotobacter spp. on the vegetative growth of Lycopersicon esculentum Mill. "Tomato" as an alternative to the indiscriminate use of chemical fertilizers. Samples were taken from roots and rhizosphere soil of vegetables from which dilutions (10-4) in Ashby-sucrose broth and incubated at 30 °C to observe a yellow color, turbidity and surface film. The genus Azotobacter was identified in mineral agar without nitrogen and Ashby-benzoate, obtaining 96 strains with a yield of 7.10 to 57.99 mgL-1 indoleacetic acid, 0.13 to 1.64 mgL-1 of fixed nitrogen as ammonium and up to 1.61% efficiency in the solubilization of phosphate Bayovar rock. It obtained a cell suspension (108) of each of the four strains with the highest values and inoculated independently and in consortium, as well as a combination with 50% urea-100 % phosphate rock in the rhizosphere of Rio Grande tomato, in a completely randomized design. The entire native strains increased height, root volume, total dry matter, shoot and root, compared with absolute control.
publishDate 2011
dc.date.none.fl_str_mv 2011-02-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://revistas.unitru.edu.pe/index.php/scientiaagrop/article/view/37
10.17268/sci.agropecu.2011.01.05
url http://revistas.unitru.edu.pe/index.php/scientiaagrop/article/view/37
identifier_str_mv 10.17268/sci.agropecu.2011.01.05
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv http://revistas.unitru.edu.pe/index.php/scientiaagrop/article/view/37/53
dc.rights.none.fl_str_mv Derechos de autor 2011 Scientia Agropecuaria
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Derechos de autor 2011 Scientia Agropecuaria
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidad Nacional de Trujillo
publisher.none.fl_str_mv Universidad Nacional de Trujillo
dc.source.none.fl_str_mv Scientia Agropecuaria; Vol. 2 No. 1 (2011): January - March; 39-49
Scientia Agropecuaria; Vol. 2 Núm. 1 (2011): Enero - Marzo; 39-49
2306-6741
2077-9917
reponame:Revista UNITRU - Scientia Agropecuaria
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reponame_str Revista UNITRU - Scientia Agropecuaria
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instname_str Universidad Nacional de Trujillo
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institution UNITRU
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repository.mail.fl_str_mv mail@mail.com
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