Induction of the acrosomal reaction in mouse spermatozoa by the solution of pellucid zone from alpaca (Lama pacos L.)

• Autores: Guzmán, Luis, Pérez, Susan, Valdivia, Martha
• Formato: artículo
• Fecha de Publicación: 2006
• Institución: Universidad Nacional Mayor de San Marcos
• Repositorio: Revista UNMSM - Revista Peruana de Biología
• Lenguaje: español
• OAI Identifier: oai:ojs.csi.unmsm:article/2351
• Enlace del recurso: https://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/2351
• Nivel de acceso: acceso abierto
• Materia: sperm capacitation; acrosome reaction; pellucid zone; Capacitación espermática; reacción acrosómica; zona pelúcida

Acrosin is one of the principal components in the acrosome, has trypsin-like activity and is secreted after the Acrosome Reaction (RA). The aim of this study was to study the induction of the acrosomal reaction in mouse sperms with homologous- Pellucid Zone and heterologous- Pellucid Zone. Mouse sperms were capacitated by two hours in IVF medium, supplemented with albumin, heparin and synthetic serum to 37 °C and 5% CO2 , and before they were incubated in absence of ZP and in present of solubilized ZP from mouse (0,78 mg/ml) and alpaca (0,78 mg/ml y 2,35 mg/ml). The RA was evaluated through immunocytochemistry with monoclonal antibodies anti-human acrosin C5F10 within intervals of 1 hour during four hours. The results show a signifi cant increase (p<0,05) in acrosome reaction induced with homologous-ZP and heterologous-ZP.