Variations of the biochemical composition of the alpaca follicular according to the growth status of the ovarian follicle

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The concentrations of total proteins, glucose, cholesterol, triglycerides, phospholipids, calcium, phosphorus, magnesium, alkaline phosphatase (ALP), aspartate transaminase (AST), alanine transaminase (ALT) and 17-β-estradiol in the follicular fluid were determined in non-pregnant Huacaya alpacas in...

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Autores: Pacheco, Joel Iván, Coila, Pedro U., Olivera, Macoly, Quispe, Andrés
Formato: artículo
Fecha de Publicación:2020
Institución:Universidad Nacional Mayor de San Marcos
Repositorio:Revista UNMSM - Revista de Investigaciones Veterinarias del Perú
Lenguaje:español
OAI Identifier:oai:ojs.csi.unmsm:article/17193
Enlace del recurso:https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/17193
Nivel de acceso:acceso abierto
Materia:alpaca
follicular fluid
biochemical composition
follicular growth
fluido folicular
composición bioquímica
crecimiento folicular
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repository_id_str .
network_name_str Revista UNMSM - Revista de Investigaciones Veterinarias del Perú
dc.title.none.fl_str_mv Variations of the biochemical composition of the alpaca follicular according to the growth status of the ovarian follicle
Variaciones de la composición bioquímica del fluido folicular de la alpaca según el estado de crecimiento del folículo ovárico
title Variations of the biochemical composition of the alpaca follicular according to the growth status of the ovarian follicle
spellingShingle Variations of the biochemical composition of the alpaca follicular according to the growth status of the ovarian follicle
Pacheco, Joel Iván
alpaca
follicular fluid
biochemical composition
follicular growth
alpaca
fluido folicular
composición bioquímica
crecimiento folicular
title_short Variations of the biochemical composition of the alpaca follicular according to the growth status of the ovarian follicle
title_full Variations of the biochemical composition of the alpaca follicular according to the growth status of the ovarian follicle
title_fullStr Variations of the biochemical composition of the alpaca follicular according to the growth status of the ovarian follicle
title_full_unstemmed Variations of the biochemical composition of the alpaca follicular according to the growth status of the ovarian follicle
title_sort Variations of the biochemical composition of the alpaca follicular according to the growth status of the ovarian follicle
dc.creator.none.fl_str_mv Pacheco, Joel Iván
Coila, Pedro U.
Olivera, Macoly
Quispe, Andrés
author Pacheco, Joel Iván
author_facet Pacheco, Joel Iván
Coila, Pedro U.
Olivera, Macoly
Quispe, Andrés
author_role author
author2 Coila, Pedro U.
Olivera, Macoly
Quispe, Andrés
author2_role author
author
author
dc.subject.none.fl_str_mv alpaca
follicular fluid
biochemical composition
follicular growth
alpaca
fluido folicular
composición bioquímica
crecimiento folicular
topic alpaca
follicular fluid
biochemical composition
follicular growth
alpaca
fluido folicular
composición bioquímica
crecimiento folicular
dc.description.none.fl_txt_mv The concentrations of total proteins, glucose, cholesterol, triglycerides, phospholipids, calcium, phosphorus, magnesium, alkaline phosphatase (ALP), aspartate transaminase (AST), alanine transaminase (ALT) and 17-β-estradiol in the follicular fluid were determined in non-pregnant Huacaya alpacas in reproductive age according to the development of the ovarian follicle (size). In total, 60 slaughtered alpacas in the local slaughterhouse were used. Samples of small (<4 mm), medium (5-7 mm) and large (>7 mm) follicles were taken (n = 20 by size). The diameter of the follicles was measured with a vernier and the follicular fluid was aspirated. The samples were analysed in the Biochemistry Laboratory of the Universidad Nacional del Altiplano (Puno, Peru) using colorimetric and spectrophotometric techniques. The concentration results for small, medium and large follicles were: total proteins (5.03, 5.68 and 7.25 g/dl), glucose (45.8, 56.4 and 69.0 mg/dl), cholesterol (3.96, 5.33 and 6.18 mg/dl) , triglycerides (17.91, 14.91 and 12.0 mg/dl), phospholipids (8.99, 6.0 and 6.11 mg/l), calcium (5.76, 8.19 and 9.58 mg/dl), phosphorus (15.69, 10.61 and 10.71 mg/dl), magnesium (2.91, 2.85 and 2.59 mg/dl), ALP (84.6, 75.4 and 75.59 IU/l), AST (238.17, 174.83 and 152.06 IU/l), ALT (24.24, 18.0 and 11.72 IU/l) and 17-β -oestradiol (0.80, 1.91 and 2.39 ng/ml). It is concluded that calcium levels increase, and levels of magnesium and alkaline phosphatase decrease as the oocyte develops, while phosphorus and phospholipid levels decrease from small follicles to medium follicles, then slightly increase in large follicles. Moreover, oestradiol content is directly related to its size.
Se determinaron las concentraciones de proteínas totales, glucosa, colesterol, triglicéridos, fosfolípidos, calcio, fósforo, magnesio, fosfatasa alcalina (ALP), aspartato transaminasa (AST), alanina transaminasa (ALT) y 17-β-estradiol en el líquido de folículos ováricos de alpacas Huacaya vacías en edad reproductiva de acuerdo con el desarrollo del folículo ovárico (tamaño). Se utilizaron 60 alpacas beneficiadas en el camal local y se tomaron muestras de folículos pequeños (<4 mm), medianos (5-7 mm) y grandes (>7 mm) (n=20 por tamaño). El diámetro de los folículos se midió con un calibrador vernier y el líquido folicular fue aspirado. Los análisis de las muestras se realizaron en el Laboratorio de Bioquímica de la Universidad Nacional del Altiplano (Puno, Perú) utilizando técnicas colorimétricas y espectrofotométricas. Los resultados de concentración para los folículos pequeños, medianos y grandes fueron: proteínas totales (5.03, 5.68 y 7.25 g/dl), glucosa (45.8, 56.4 y 69.0 mg/dl), colesterol (3.96, 5.33 y 6.18 mg/dl), triglicéridos (17.91, 14.91 y 12.0 mg/dl), fosfolípidos (8.99, 6.0 y 6.11 mg/l), calcio (5.76, 8.19 y 9.58 mg/dl), fósforo (15.69, 10.61 y 10.71 mg/dl), magnesio (2.91, 2.85 y 2.59 mg/dl), ALP (84.6, 75.4 y 75.59 UI/l), AST (238.17, 174.83 y 152.06 UI/l), ALT (24.24, 18.0 y 11.72 UI/l) y 17-β-estradiol (0.80, 1.91 y 2.39 ng/ml). Se concluye que los niveles de calcio se incrementan y los niveles de magnesio y fosfatasa alcalina disminuyen conforme se desarrolla el ovocito, mientras que los niveles de fósforo y fosfolípidos disminuyen de folículos pequeños a folículos medianos, para luego incrementarse ligeramente en folículos grandes. Además, el contenido de estradiol está en relación directa con su tamaño.
description The concentrations of total proteins, glucose, cholesterol, triglycerides, phospholipids, calcium, phosphorus, magnesium, alkaline phosphatase (ALP), aspartate transaminase (AST), alanine transaminase (ALT) and 17-β-estradiol in the follicular fluid were determined in non-pregnant Huacaya alpacas in reproductive age according to the development of the ovarian follicle (size). In total, 60 slaughtered alpacas in the local slaughterhouse were used. Samples of small (<4 mm), medium (5-7 mm) and large (>7 mm) follicles were taken (n = 20 by size). The diameter of the follicles was measured with a vernier and the follicular fluid was aspirated. The samples were analysed in the Biochemistry Laboratory of the Universidad Nacional del Altiplano (Puno, Peru) using colorimetric and spectrophotometric techniques. The concentration results for small, medium and large follicles were: total proteins (5.03, 5.68 and 7.25 g/dl), glucose (45.8, 56.4 and 69.0 mg/dl), cholesterol (3.96, 5.33 and 6.18 mg/dl) , triglycerides (17.91, 14.91 and 12.0 mg/dl), phospholipids (8.99, 6.0 and 6.11 mg/l), calcium (5.76, 8.19 and 9.58 mg/dl), phosphorus (15.69, 10.61 and 10.71 mg/dl), magnesium (2.91, 2.85 and 2.59 mg/dl), ALP (84.6, 75.4 and 75.59 IU/l), AST (238.17, 174.83 and 152.06 IU/l), ALT (24.24, 18.0 and 11.72 IU/l) and 17-β -oestradiol (0.80, 1.91 and 2.39 ng/ml). It is concluded that calcium levels increase, and levels of magnesium and alkaline phosphatase decrease as the oocyte develops, while phosphorus and phospholipid levels decrease from small follicles to medium follicles, then slightly increase in large follicles. Moreover, oestradiol content is directly related to its size.
publishDate 2020
dc.date.none.fl_str_mv 2020-02-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/17193
10.15381/rivep.v30i4.17193
url https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/17193
identifier_str_mv 10.15381/rivep.v30i4.17193
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/17193/14621
dc.rights.none.fl_str_mv Derechos de autor 2020 Joel Iván Pacheco, Pedro U. Coila, Macoly Olivera, Andrés Quispe
http://creativecommons.org/licenses/by-nc-sa/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Derechos de autor 2020 Joel Iván Pacheco, Pedro U. Coila, Macoly Olivera, Andrés Quispe
http://creativecommons.org/licenses/by-nc-sa/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria
publisher.none.fl_str_mv Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria
dc.source.none.fl_str_mv Revista de Investigaciones Veterinarias del Perú; Vol 30 No 4 (2019); 1629-1636
Revista de Investigaciones Veterinarias del Perú; Vol. 30 Núm. 4 (2019); 1629-1636
1682-3419
1609-9117
reponame:Revista UNMSM - Revista de Investigaciones Veterinarias del Perú
instname:Universidad Nacional Mayor de San Marcos
instacron:UNMSM
reponame_str Revista UNMSM - Revista de Investigaciones Veterinarias del Perú
collection Revista UNMSM - Revista de Investigaciones Veterinarias del Perú
instname_str Universidad Nacional Mayor de San Marcos
instacron_str UNMSM
institution UNMSM
repository.name.fl_str_mv -
repository.mail.fl_str_mv mail@mail.com
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spelling Variations of the biochemical composition of the alpaca follicular according to the growth status of the ovarian follicleVariaciones de la composición bioquímica del fluido folicular de la alpaca según el estado de crecimiento del folículo ováricoPacheco, Joel IvánCoila, Pedro U.Olivera, MacolyQuispe, Andrésalpacafollicular fluidbiochemical compositionfollicular growthalpacafluido folicularcomposición bioquímicacrecimiento folicularThe concentrations of total proteins, glucose, cholesterol, triglycerides, phospholipids, calcium, phosphorus, magnesium, alkaline phosphatase (ALP), aspartate transaminase (AST), alanine transaminase (ALT) and 17-β-estradiol in the follicular fluid were determined in non-pregnant Huacaya alpacas in reproductive age according to the development of the ovarian follicle (size). In total, 60 slaughtered alpacas in the local slaughterhouse were used. Samples of small (<4 mm), medium (5-7 mm) and large (>7 mm) follicles were taken (n = 20 by size). The diameter of the follicles was measured with a vernier and the follicular fluid was aspirated. The samples were analysed in the Biochemistry Laboratory of the Universidad Nacional del Altiplano (Puno, Peru) using colorimetric and spectrophotometric techniques. The concentration results for small, medium and large follicles were: total proteins (5.03, 5.68 and 7.25 g/dl), glucose (45.8, 56.4 and 69.0 mg/dl), cholesterol (3.96, 5.33 and 6.18 mg/dl) , triglycerides (17.91, 14.91 and 12.0 mg/dl), phospholipids (8.99, 6.0 and 6.11 mg/l), calcium (5.76, 8.19 and 9.58 mg/dl), phosphorus (15.69, 10.61 and 10.71 mg/dl), magnesium (2.91, 2.85 and 2.59 mg/dl), ALP (84.6, 75.4 and 75.59 IU/l), AST (238.17, 174.83 and 152.06 IU/l), ALT (24.24, 18.0 and 11.72 IU/l) and 17-β -oestradiol (0.80, 1.91 and 2.39 ng/ml). It is concluded that calcium levels increase, and levels of magnesium and alkaline phosphatase decrease as the oocyte develops, while phosphorus and phospholipid levels decrease from small follicles to medium follicles, then slightly increase in large follicles. Moreover, oestradiol content is directly related to its size.Se determinaron las concentraciones de proteínas totales, glucosa, colesterol, triglicéridos, fosfolípidos, calcio, fósforo, magnesio, fosfatasa alcalina (ALP), aspartato transaminasa (AST), alanina transaminasa (ALT) y 17-β-estradiol en el líquido de folículos ováricos de alpacas Huacaya vacías en edad reproductiva de acuerdo con el desarrollo del folículo ovárico (tamaño). Se utilizaron 60 alpacas beneficiadas en el camal local y se tomaron muestras de folículos pequeños (<4 mm), medianos (5-7 mm) y grandes (>7 mm) (n=20 por tamaño). El diámetro de los folículos se midió con un calibrador vernier y el líquido folicular fue aspirado. Los análisis de las muestras se realizaron en el Laboratorio de Bioquímica de la Universidad Nacional del Altiplano (Puno, Perú) utilizando técnicas colorimétricas y espectrofotométricas. Los resultados de concentración para los folículos pequeños, medianos y grandes fueron: proteínas totales (5.03, 5.68 y 7.25 g/dl), glucosa (45.8, 56.4 y 69.0 mg/dl), colesterol (3.96, 5.33 y 6.18 mg/dl), triglicéridos (17.91, 14.91 y 12.0 mg/dl), fosfolípidos (8.99, 6.0 y 6.11 mg/l), calcio (5.76, 8.19 y 9.58 mg/dl), fósforo (15.69, 10.61 y 10.71 mg/dl), magnesio (2.91, 2.85 y 2.59 mg/dl), ALP (84.6, 75.4 y 75.59 UI/l), AST (238.17, 174.83 y 152.06 UI/l), ALT (24.24, 18.0 y 11.72 UI/l) y 17-β-estradiol (0.80, 1.91 y 2.39 ng/ml). Se concluye que los niveles de calcio se incrementan y los niveles de magnesio y fosfatasa alcalina disminuyen conforme se desarrolla el ovocito, mientras que los niveles de fósforo y fosfolípidos disminuyen de folículos pequeños a folículos medianos, para luego incrementarse ligeramente en folículos grandes. Además, el contenido de estradiol está en relación directa con su tamaño.Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria2020-02-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/1719310.15381/rivep.v30i4.17193Revista de Investigaciones Veterinarias del Perú; Vol 30 No 4 (2019); 1629-1636Revista de Investigaciones Veterinarias del Perú; Vol. 30 Núm. 4 (2019); 1629-16361682-34191609-9117reponame:Revista UNMSM - Revista de Investigaciones Veterinarias del Perúinstname:Universidad Nacional Mayor de San Marcosinstacron:UNMSMspahttps://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/17193/14621Derechos de autor 2020 Joel Iván Pacheco, Pedro U. Coila, Macoly Olivera, Andrés Quispehttp://creativecommons.org/licenses/by-nc-sa/4.0info:eu-repo/semantics/openAccess2021-06-01T18:10:12Zmail@mail.com -
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