Detection and association of Piscirickettsia salmonis in organ and faeces of coho salmon (Oncorhynchus kisutch) farmed in the sea

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The aim of this study was to diagnose the presence of Piscirickettsia salmonis in samples of coho salmon (Oncorhynchus kisutch) from fish farms in the Los Lagos Region, Chile, which presented outbreaks of piscirickettsiosis. In total, 19 fish with clinical signs of piscirickettsiosis were used. Smea...

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Detalles Bibliográficos
Autores: Montalico Pongo, Ederson Juan, Rodríguez Papuico, Héctor, Larenas Herrera, Julio, Ardito Saenz, Fernando Ricardo
Formato: artículo
Fecha de Publicación:2021
Institución:Universidad Nacional Mayor de San Marcos
Repositorio:Revista UNMSM - Revista de Investigaciones Veterinarias del Perú
Lenguaje:español
OAI Identifier:oai:ojs.csi.unmsm:article/18041
Enlace del recurso:https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/18041
Nivel de acceso:acceso abierto
Materia:indirect immunofluorescence
piscirickettsiosis
Salmonidae
inmunofluorescencia indirecta
Piscirickettsiaceae
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spelling Detection and association of Piscirickettsia salmonis in organ and faeces of coho salmon (Oncorhynchus kisutch) farmed in the seaDetección y asociación de Piscirickettsia salmonis en órganos y heces de salmón coho (Oncorhynchus kisutch) cultivado en el marMontalico Pongo, Ederson JuanRodríguez Papuico, HéctorLarenas Herrera, JulioArdito Saenz, Fernando Ricardoindirect immunofluorescencepiscirickettsiosisSalmonidaeinmunofluorescencia indirectaPiscirickettsiaceaeSalmonidaeThe aim of this study was to diagnose the presence of Piscirickettsia salmonis in samples of coho salmon (Oncorhynchus kisutch) from fish farms in the Los Lagos Region, Chile, which presented outbreaks of piscirickettsiosis. In total, 19 fish with clinical signs of piscirickettsiosis were used. Smears of kidney, liver and splenic tissue and intestinal content were made, and processed using the indirect immunofluorescence test (IFAT) for the detection of P. salmonis through a commercial kit. The data obtained were subjected to the Cochran and McNemar Q test in the Chi square distribution. Higher positivity was found in the liver (68.4%) and kidney (52.6%). In addition, the bacterium was found in the faeces (47.4%), indicating that the elimination via the faeces of the agent is possible under farming conditions. By associating the kidney and liver results, only 78.9% of the fish positive for the bacteria could be detected, while by associating the liver, spleen and faeces results, 100% of the fish positive to P. salmonis can be detected.El objetivo de la investigación fue diagnosticar la presencia de Piscirickettsia salmonis en muestras de salmones coho (Oncorhynchus kisutch) provenientes de centros de cultivo de la Región de los Lagos, Chile, que presentaban brotes de piscirickettsiosis. Se emplearon 19 peces con signos clínicos de piscirickettsiosis. Se hicieron frotis de tejido renal, hepático, esplénico y de contenido intestinal que se procesaron mediante la prueba de inmunofluorescencia indirecta (IFI) para la detección de P. salmonis a través de un kit comercial. Los datos obtenidos se sometieron a la prueba de Q de Cochran y de McNemar en la distribución de Chi cuadrado. Se encontró mayor positividad en el hígado (68.4%) y riñón (52.6%). Además, se encontró la bacteria en las heces (47.4%), indicando que la eliminación vía fecal del agente es posible en condiciones de cultivo. Asociando los resultados de riñón e hígado solo de logra detectar el 78.9% de los peces positivos a la bacteria, en tanto que asociando los resultados de hígado, bazo y heces se logra detectar el 100% de los peces positivos a P. salmonis.Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria2021-04-22info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/1804110.15381/rivep.v32i2.18041Revista de Investigaciones Veterinarias del Perú; Vol 32 No 2 (2021); e18041Revista de Investigaciones Veterinarias del Perú; Vol. 32 Núm. 2 (2021); e180411682-34191609-9117reponame:Revista UNMSM - Revista de Investigaciones Veterinarias del Perúinstname:Universidad Nacional Mayor de San Marcosinstacron:UNMSMspahttps://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/18041/16546Derechos de autor 2021 Ederson Juan Montalico Pongo, Héctor Rodríguez Papuico, Julio Larenas Herrera, Fernando Ricardo Ardito Saenzhttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccess2021-06-01T18:11:00Zmail@mail.com -
dc.title.none.fl_str_mv Detection and association of Piscirickettsia salmonis in organ and faeces of coho salmon (Oncorhynchus kisutch) farmed in the sea
Detección y asociación de Piscirickettsia salmonis en órganos y heces de salmón coho (Oncorhynchus kisutch) cultivado en el mar
title Detection and association of Piscirickettsia salmonis in organ and faeces of coho salmon (Oncorhynchus kisutch) farmed in the sea
spellingShingle Detection and association of Piscirickettsia salmonis in organ and faeces of coho salmon (Oncorhynchus kisutch) farmed in the sea
Montalico Pongo, Ederson Juan
indirect immunofluorescence
piscirickettsiosis
Salmonidae
inmunofluorescencia indirecta
Piscirickettsiaceae
Salmonidae
title_short Detection and association of Piscirickettsia salmonis in organ and faeces of coho salmon (Oncorhynchus kisutch) farmed in the sea
title_full Detection and association of Piscirickettsia salmonis in organ and faeces of coho salmon (Oncorhynchus kisutch) farmed in the sea
title_fullStr Detection and association of Piscirickettsia salmonis in organ and faeces of coho salmon (Oncorhynchus kisutch) farmed in the sea
title_full_unstemmed Detection and association of Piscirickettsia salmonis in organ and faeces of coho salmon (Oncorhynchus kisutch) farmed in the sea
title_sort Detection and association of Piscirickettsia salmonis in organ and faeces of coho salmon (Oncorhynchus kisutch) farmed in the sea
dc.creator.none.fl_str_mv Montalico Pongo, Ederson Juan
Rodríguez Papuico, Héctor
Larenas Herrera, Julio
Ardito Saenz, Fernando Ricardo
author Montalico Pongo, Ederson Juan
author_facet Montalico Pongo, Ederson Juan
Rodríguez Papuico, Héctor
Larenas Herrera, Julio
Ardito Saenz, Fernando Ricardo
author_role author
author2 Rodríguez Papuico, Héctor
Larenas Herrera, Julio
Ardito Saenz, Fernando Ricardo
author2_role author
author
author
dc.subject.none.fl_str_mv indirect immunofluorescence
piscirickettsiosis
Salmonidae
inmunofluorescencia indirecta
Piscirickettsiaceae
Salmonidae
topic indirect immunofluorescence
piscirickettsiosis
Salmonidae
inmunofluorescencia indirecta
Piscirickettsiaceae
Salmonidae
dc.description.none.fl_txt_mv The aim of this study was to diagnose the presence of Piscirickettsia salmonis in samples of coho salmon (Oncorhynchus kisutch) from fish farms in the Los Lagos Region, Chile, which presented outbreaks of piscirickettsiosis. In total, 19 fish with clinical signs of piscirickettsiosis were used. Smears of kidney, liver and splenic tissue and intestinal content were made, and processed using the indirect immunofluorescence test (IFAT) for the detection of P. salmonis through a commercial kit. The data obtained were subjected to the Cochran and McNemar Q test in the Chi square distribution. Higher positivity was found in the liver (68.4%) and kidney (52.6%). In addition, the bacterium was found in the faeces (47.4%), indicating that the elimination via the faeces of the agent is possible under farming conditions. By associating the kidney and liver results, only 78.9% of the fish positive for the bacteria could be detected, while by associating the liver, spleen and faeces results, 100% of the fish positive to P. salmonis can be detected.
El objetivo de la investigación fue diagnosticar la presencia de Piscirickettsia salmonis en muestras de salmones coho (Oncorhynchus kisutch) provenientes de centros de cultivo de la Región de los Lagos, Chile, que presentaban brotes de piscirickettsiosis. Se emplearon 19 peces con signos clínicos de piscirickettsiosis. Se hicieron frotis de tejido renal, hepático, esplénico y de contenido intestinal que se procesaron mediante la prueba de inmunofluorescencia indirecta (IFI) para la detección de P. salmonis a través de un kit comercial. Los datos obtenidos se sometieron a la prueba de Q de Cochran y de McNemar en la distribución de Chi cuadrado. Se encontró mayor positividad en el hígado (68.4%) y riñón (52.6%). Además, se encontró la bacteria en las heces (47.4%), indicando que la eliminación vía fecal del agente es posible en condiciones de cultivo. Asociando los resultados de riñón e hígado solo de logra detectar el 78.9% de los peces positivos a la bacteria, en tanto que asociando los resultados de hígado, bazo y heces se logra detectar el 100% de los peces positivos a P. salmonis.
description The aim of this study was to diagnose the presence of Piscirickettsia salmonis in samples of coho salmon (Oncorhynchus kisutch) from fish farms in the Los Lagos Region, Chile, which presented outbreaks of piscirickettsiosis. In total, 19 fish with clinical signs of piscirickettsiosis were used. Smears of kidney, liver and splenic tissue and intestinal content were made, and processed using the indirect immunofluorescence test (IFAT) for the detection of P. salmonis through a commercial kit. The data obtained were subjected to the Cochran and McNemar Q test in the Chi square distribution. Higher positivity was found in the liver (68.4%) and kidney (52.6%). In addition, the bacterium was found in the faeces (47.4%), indicating that the elimination via the faeces of the agent is possible under farming conditions. By associating the kidney and liver results, only 78.9% of the fish positive for the bacteria could be detected, while by associating the liver, spleen and faeces results, 100% of the fish positive to P. salmonis can be detected.
publishDate 2021
dc.date.none.fl_str_mv 2021-04-22
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/18041
10.15381/rivep.v32i2.18041
url https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/18041
identifier_str_mv 10.15381/rivep.v32i2.18041
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/18041/16546
dc.rights.none.fl_str_mv Derechos de autor 2021 Ederson Juan Montalico Pongo, Héctor Rodríguez Papuico, Julio Larenas Herrera, Fernando Ricardo Ardito Saenz
http://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Derechos de autor 2021 Ederson Juan Montalico Pongo, Héctor Rodríguez Papuico, Julio Larenas Herrera, Fernando Ricardo Ardito Saenz
http://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria
publisher.none.fl_str_mv Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria
dc.source.none.fl_str_mv Revista de Investigaciones Veterinarias del Perú; Vol 32 No 2 (2021); e18041
Revista de Investigaciones Veterinarias del Perú; Vol. 32 Núm. 2 (2021); e18041
1682-3419
1609-9117
reponame:Revista UNMSM - Revista de Investigaciones Veterinarias del Perú
instname:Universidad Nacional Mayor de San Marcos
instacron:UNMSM
reponame_str Revista UNMSM - Revista de Investigaciones Veterinarias del Perú
collection Revista UNMSM - Revista de Investigaciones Veterinarias del Perú
instname_str Universidad Nacional Mayor de San Marcos
instacron_str UNMSM
institution UNMSM
repository.name.fl_str_mv -
repository.mail.fl_str_mv mail@mail.com
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score 13.905282
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