Detection and association of Piscirickettsia salmonis in organ and faeces of coho salmon (Oncorhynchus kisutch) farmed in the sea
Descripción del Articulo
The aim of this study was to diagnose the presence of Piscirickettsia salmonis in samples of coho salmon (Oncorhynchus kisutch) from fish farms in the Los Lagos Region, Chile, which presented outbreaks of piscirickettsiosis. In total, 19 fish with clinical signs of piscirickettsiosis were used. Smea...
| Autores: | , , , |
|---|---|
| Formato: | artículo |
| Fecha de Publicación: | 2021 |
| Institución: | Universidad Nacional Mayor de San Marcos |
| Repositorio: | Revista UNMSM - Revista de Investigaciones Veterinarias del Perú |
| Lenguaje: | español |
| OAI Identifier: | oai:ojs.csi.unmsm:article/18041 |
| Enlace del recurso: | https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/18041 |
| Nivel de acceso: | acceso abierto |
| Materia: | indirect immunofluorescence piscirickettsiosis Salmonidae inmunofluorescencia indirecta Piscirickettsiaceae |
| id |
1609-9117_4de45f18698986666cc60a76003d4cc4 |
|---|---|
| oai_identifier_str |
oai:ojs.csi.unmsm:article/18041 |
| network_acronym_str |
1609-9117 |
| repository_id_str |
. |
| network_name_str |
Revista UNMSM - Revista de Investigaciones Veterinarias del Perú |
| spelling |
Detection and association of Piscirickettsia salmonis in organ and faeces of coho salmon (Oncorhynchus kisutch) farmed in the seaDetección y asociación de Piscirickettsia salmonis en órganos y heces de salmón coho (Oncorhynchus kisutch) cultivado en el marMontalico Pongo, Ederson JuanRodríguez Papuico, HéctorLarenas Herrera, JulioArdito Saenz, Fernando Ricardoindirect immunofluorescencepiscirickettsiosisSalmonidaeinmunofluorescencia indirectaPiscirickettsiaceaeSalmonidaeThe aim of this study was to diagnose the presence of Piscirickettsia salmonis in samples of coho salmon (Oncorhynchus kisutch) from fish farms in the Los Lagos Region, Chile, which presented outbreaks of piscirickettsiosis. In total, 19 fish with clinical signs of piscirickettsiosis were used. Smears of kidney, liver and splenic tissue and intestinal content were made, and processed using the indirect immunofluorescence test (IFAT) for the detection of P. salmonis through a commercial kit. The data obtained were subjected to the Cochran and McNemar Q test in the Chi square distribution. Higher positivity was found in the liver (68.4%) and kidney (52.6%). In addition, the bacterium was found in the faeces (47.4%), indicating that the elimination via the faeces of the agent is possible under farming conditions. By associating the kidney and liver results, only 78.9% of the fish positive for the bacteria could be detected, while by associating the liver, spleen and faeces results, 100% of the fish positive to P. salmonis can be detected.El objetivo de la investigación fue diagnosticar la presencia de Piscirickettsia salmonis en muestras de salmones coho (Oncorhynchus kisutch) provenientes de centros de cultivo de la Región de los Lagos, Chile, que presentaban brotes de piscirickettsiosis. Se emplearon 19 peces con signos clínicos de piscirickettsiosis. Se hicieron frotis de tejido renal, hepático, esplénico y de contenido intestinal que se procesaron mediante la prueba de inmunofluorescencia indirecta (IFI) para la detección de P. salmonis a través de un kit comercial. Los datos obtenidos se sometieron a la prueba de Q de Cochran y de McNemar en la distribución de Chi cuadrado. Se encontró mayor positividad en el hígado (68.4%) y riñón (52.6%). Además, se encontró la bacteria en las heces (47.4%), indicando que la eliminación vía fecal del agente es posible en condiciones de cultivo. Asociando los resultados de riñón e hígado solo de logra detectar el 78.9% de los peces positivos a la bacteria, en tanto que asociando los resultados de hígado, bazo y heces se logra detectar el 100% de los peces positivos a P. salmonis.Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria2021-04-22info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/1804110.15381/rivep.v32i2.18041Revista de Investigaciones Veterinarias del Perú; Vol 32 No 2 (2021); e18041Revista de Investigaciones Veterinarias del Perú; Vol. 32 Núm. 2 (2021); e180411682-34191609-9117reponame:Revista UNMSM - Revista de Investigaciones Veterinarias del Perúinstname:Universidad Nacional Mayor de San Marcosinstacron:UNMSMspahttps://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/18041/16546Derechos de autor 2021 Ederson Juan Montalico Pongo, Héctor Rodríguez Papuico, Julio Larenas Herrera, Fernando Ricardo Ardito Saenzhttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccess2021-06-01T18:11:00Zmail@mail.com - |
| dc.title.none.fl_str_mv |
Detection and association of Piscirickettsia salmonis in organ and faeces of coho salmon (Oncorhynchus kisutch) farmed in the sea Detección y asociación de Piscirickettsia salmonis en órganos y heces de salmón coho (Oncorhynchus kisutch) cultivado en el mar |
| title |
Detection and association of Piscirickettsia salmonis in organ and faeces of coho salmon (Oncorhynchus kisutch) farmed in the sea |
| spellingShingle |
Detection and association of Piscirickettsia salmonis in organ and faeces of coho salmon (Oncorhynchus kisutch) farmed in the sea Montalico Pongo, Ederson Juan indirect immunofluorescence piscirickettsiosis Salmonidae inmunofluorescencia indirecta Piscirickettsiaceae Salmonidae |
| title_short |
Detection and association of Piscirickettsia salmonis in organ and faeces of coho salmon (Oncorhynchus kisutch) farmed in the sea |
| title_full |
Detection and association of Piscirickettsia salmonis in organ and faeces of coho salmon (Oncorhynchus kisutch) farmed in the sea |
| title_fullStr |
Detection and association of Piscirickettsia salmonis in organ and faeces of coho salmon (Oncorhynchus kisutch) farmed in the sea |
| title_full_unstemmed |
Detection and association of Piscirickettsia salmonis in organ and faeces of coho salmon (Oncorhynchus kisutch) farmed in the sea |
| title_sort |
Detection and association of Piscirickettsia salmonis in organ and faeces of coho salmon (Oncorhynchus kisutch) farmed in the sea |
| dc.creator.none.fl_str_mv |
Montalico Pongo, Ederson Juan Rodríguez Papuico, Héctor Larenas Herrera, Julio Ardito Saenz, Fernando Ricardo |
| author |
Montalico Pongo, Ederson Juan |
| author_facet |
Montalico Pongo, Ederson Juan Rodríguez Papuico, Héctor Larenas Herrera, Julio Ardito Saenz, Fernando Ricardo |
| author_role |
author |
| author2 |
Rodríguez Papuico, Héctor Larenas Herrera, Julio Ardito Saenz, Fernando Ricardo |
| author2_role |
author author author |
| dc.subject.none.fl_str_mv |
indirect immunofluorescence piscirickettsiosis Salmonidae inmunofluorescencia indirecta Piscirickettsiaceae Salmonidae |
| topic |
indirect immunofluorescence piscirickettsiosis Salmonidae inmunofluorescencia indirecta Piscirickettsiaceae Salmonidae |
| dc.description.none.fl_txt_mv |
The aim of this study was to diagnose the presence of Piscirickettsia salmonis in samples of coho salmon (Oncorhynchus kisutch) from fish farms in the Los Lagos Region, Chile, which presented outbreaks of piscirickettsiosis. In total, 19 fish with clinical signs of piscirickettsiosis were used. Smears of kidney, liver and splenic tissue and intestinal content were made, and processed using the indirect immunofluorescence test (IFAT) for the detection of P. salmonis through a commercial kit. The data obtained were subjected to the Cochran and McNemar Q test in the Chi square distribution. Higher positivity was found in the liver (68.4%) and kidney (52.6%). In addition, the bacterium was found in the faeces (47.4%), indicating that the elimination via the faeces of the agent is possible under farming conditions. By associating the kidney and liver results, only 78.9% of the fish positive for the bacteria could be detected, while by associating the liver, spleen and faeces results, 100% of the fish positive to P. salmonis can be detected. El objetivo de la investigación fue diagnosticar la presencia de Piscirickettsia salmonis en muestras de salmones coho (Oncorhynchus kisutch) provenientes de centros de cultivo de la Región de los Lagos, Chile, que presentaban brotes de piscirickettsiosis. Se emplearon 19 peces con signos clínicos de piscirickettsiosis. Se hicieron frotis de tejido renal, hepático, esplénico y de contenido intestinal que se procesaron mediante la prueba de inmunofluorescencia indirecta (IFI) para la detección de P. salmonis a través de un kit comercial. Los datos obtenidos se sometieron a la prueba de Q de Cochran y de McNemar en la distribución de Chi cuadrado. Se encontró mayor positividad en el hígado (68.4%) y riñón (52.6%). Además, se encontró la bacteria en las heces (47.4%), indicando que la eliminación vía fecal del agente es posible en condiciones de cultivo. Asociando los resultados de riñón e hígado solo de logra detectar el 78.9% de los peces positivos a la bacteria, en tanto que asociando los resultados de hígado, bazo y heces se logra detectar el 100% de los peces positivos a P. salmonis. |
| description |
The aim of this study was to diagnose the presence of Piscirickettsia salmonis in samples of coho salmon (Oncorhynchus kisutch) from fish farms in the Los Lagos Region, Chile, which presented outbreaks of piscirickettsiosis. In total, 19 fish with clinical signs of piscirickettsiosis were used. Smears of kidney, liver and splenic tissue and intestinal content were made, and processed using the indirect immunofluorescence test (IFAT) for the detection of P. salmonis through a commercial kit. The data obtained were subjected to the Cochran and McNemar Q test in the Chi square distribution. Higher positivity was found in the liver (68.4%) and kidney (52.6%). In addition, the bacterium was found in the faeces (47.4%), indicating that the elimination via the faeces of the agent is possible under farming conditions. By associating the kidney and liver results, only 78.9% of the fish positive for the bacteria could be detected, while by associating the liver, spleen and faeces results, 100% of the fish positive to P. salmonis can be detected. |
| publishDate |
2021 |
| dc.date.none.fl_str_mv |
2021-04-22 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/18041 10.15381/rivep.v32i2.18041 |
| url |
https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/18041 |
| identifier_str_mv |
10.15381/rivep.v32i2.18041 |
| dc.language.none.fl_str_mv |
spa |
| language |
spa |
| dc.relation.none.fl_str_mv |
https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/18041/16546 |
| dc.rights.none.fl_str_mv |
Derechos de autor 2021 Ederson Juan Montalico Pongo, Héctor Rodríguez Papuico, Julio Larenas Herrera, Fernando Ricardo Ardito Saenz http://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
Derechos de autor 2021 Ederson Juan Montalico Pongo, Héctor Rodríguez Papuico, Julio Larenas Herrera, Fernando Ricardo Ardito Saenz http://creativecommons.org/licenses/by/4.0 |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria |
| publisher.none.fl_str_mv |
Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria |
| dc.source.none.fl_str_mv |
Revista de Investigaciones Veterinarias del Perú; Vol 32 No 2 (2021); e18041 Revista de Investigaciones Veterinarias del Perú; Vol. 32 Núm. 2 (2021); e18041 1682-3419 1609-9117 reponame:Revista UNMSM - Revista de Investigaciones Veterinarias del Perú instname:Universidad Nacional Mayor de San Marcos instacron:UNMSM |
| reponame_str |
Revista UNMSM - Revista de Investigaciones Veterinarias del Perú |
| collection |
Revista UNMSM - Revista de Investigaciones Veterinarias del Perú |
| instname_str |
Universidad Nacional Mayor de San Marcos |
| instacron_str |
UNMSM |
| institution |
UNMSM |
| repository.name.fl_str_mv |
-
|
| repository.mail.fl_str_mv |
mail@mail.com |
| _version_ |
1701389177570983936 |
| score |
13.905282 |
Nota importante:
La información contenida en este registro es de entera responsabilidad de la institución que gestiona el repositorio institucional donde esta contenido este documento o set de datos. El CONCYTEC no se hace responsable por los contenidos (publicaciones y/o datos) accesibles a través del Repositorio Nacional Digital de Ciencia, Tecnología e Innovación de Acceso Abierto (ALICIA).
La información contenida en este registro es de entera responsabilidad de la institución que gestiona el repositorio institucional donde esta contenido este documento o set de datos. El CONCYTEC no se hace responsable por los contenidos (publicaciones y/o datos) accesibles a través del Repositorio Nacional Digital de Ciencia, Tecnología e Innovación de Acceso Abierto (ALICIA).