Detection of a protein associated with acute hepatopancreatic necrosis disease (AHPND) in Litopenaeus vannamei under semi-intensive farming in Ecuador
Descripción del Articulo
The present study aimed to detect a protein associated with acute hepatopancreatic necrosis (AHPND) by mass spectrometry, reared under semi-intensive farming in Ecuador. Sick shrimps from three farms were collected in the Bellavista area in the El Oro province. The hepatopancreas were macerated and...
| Autores: | , , , , , , , , , , |
|---|---|
| Formato: | artículo |
| Fecha de Publicación: | 2018 |
| Institución: | Universidad Nacional Mayor de San Marcos |
| Repositorio: | Revistas - Universidad Nacional Mayor de San Marcos |
| Lenguaje: | español |
| OAI Identifier: | oai:ojs.csi.unmsm:article/14194 |
| Enlace del recurso: | https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/14194 |
| Nivel de acceso: | acceso abierto |
| Materia: | shrimp AHPND Litopenaus vannamei early mortality syndrome Vibrio parahaemolitycus PirvpB camarón Litopenaeus vannamei síndrome de mortalidad precoz Vibrio parahaemolyticus |
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Revistas - Universidad Nacional Mayor de San Marcos |
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Detection of a protein associated with acute hepatopancreatic necrosis disease (AHPND) in Litopenaeus vannamei under semi-intensive farming in EcuadorDetección de una proteína asociada a la enfermedad de la necrosis hepatopancreatica aguda (AHPND) en Litopenaeus vannamei bajo cultivo semi-intensivo en EcuadorSaavedra-Olivos, Katherine YulianaPeralta-Ortiz, TessyOrdinola-Zapata, AlbertoSandoval-Ramayoni, John EstuardoVieyra-Peña, Enedia GracielaZapata-Cruz, Marco AntonioHidalgo-Mogollón, AubertoMorán, BraulioMendoza-Neyra, Oscar AugustoMendoza-Dioses, Magno EgoCampoverde-Peña, Sigrid YamileshrimpAHPNDLitopenaus vannameiearly mortality syndromeVibrio parahaemolitycusPirvpBcamarónAHPNDLitopenaeus vannameisíndrome de mortalidad precozVibrio parahaemolyticusPirvpBThe present study aimed to detect a protein associated with acute hepatopancreatic necrosis (AHPND) by mass spectrometry, reared under semi-intensive farming in Ecuador. Sick shrimps from three farms were collected in the Bellavista area in the El Oro province. The hepatopancreas were macerated and cultured in TCBS medium and subcultured in TSA and LB broth. In the bacterial strains obtained, the proteins were extracted using a commercial kit and separated by SDS-PAGE gel migration. These were analyzed with a MALDI TOF/TOF mass spectrometer. The confirmation of the strains was performed by PCR using TUMSAT-Vp3 primers, which are specific for detecting AHPND. One of the strains had peptide sequences similar to that of the PirvpB protein causing AHPND, and was identified as belonging to Vibrio parahaemolyticus and carrying the gene coding for PirvpB. The results showed that it is possible to use MALDI TOF/TOF mass spectrometry in the detection of AHPND-associated proteins in shrimp culture.La presente investigación tuvo como objetivo detectar una proteína asociada a la necrosis hepatopancreática aguda (AHPND), en cultivos semi-intensivos en Ecuador. Se recolectaron camarones enfermos de tres camaroneras en la zona de Bellavista, provincia El Oro. Los hepatopáncreas fueron macerados y cultivados en medio TCBS y subcultivos en TSA y caldo LB. De las cepas bacterianas obtenidas, se extrajo las proteínas usando un kit comercial y se separaron mediante migración en gel SDS-PAGE. Estas fueron analizadas con un espectrómetro de masas MALDI TOF/TOF. La confirmación de las cepas se realizó mediante PCR utilizando cebadores TUMSAT-Vp3, que son específicos para detectar AHPND. Una de las cepas tuvo secuencias peptídicas similares a la de la proteína PirvpB, causante de AHPND, y fue identificada como perteneciente a Vibrio parahaemolyticus y portadora del gen que codifica PirvpB, por tanto, positiva para AHPND. Los resultados mostraron que es posible usar la espectrometría de masas MALDI TOF/TOF en la detección de proteínas asociadas a AHPND en el cultivo de camarón.Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria2018-03-14info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdftext/htmlhttps://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/1419410.15381/rivep.v29i1.14194Revista de Investigaciones Veterinarias del Perú; Vol. 29 Núm. 1 (2018); 328-338Revista de Investigaciones Veterinarias del Perú; Vol. 29 No. 1 (2018); 328-3381682-34191609-9117reponame:Revistas - Universidad Nacional Mayor de San Marcosinstname:Universidad Nacional Mayor de San Marcosinstacron:UNMSMspahttps://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/14194/12767https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/14194/14007Derechos de autor 2018 Katherine Yuliana Saavedra-Olivos, Tessy Peralta-Ortiz, Alberto Ordinola-Zapata, John Estuardo Sandoval-Ramayoni, Enedia Graciela Vieyra-Peña, Marco Antonio Zapata-Cruz, Auberto Hidalgo-Mogollón, Braulio Morán, Oscar Augusto Mendoza-Neyra, Magno Ego Mendoza-Dioses, Sigrid Yamile Campoverde-Peñahttps://creativecommons.org/licenses/by-nc-sa/4.0info:eu-repo/semantics/openAccessoai:ojs.csi.unmsm:article/141942018-03-15T04:44:35Z |
| dc.title.none.fl_str_mv |
Detection of a protein associated with acute hepatopancreatic necrosis disease (AHPND) in Litopenaeus vannamei under semi-intensive farming in Ecuador Detección de una proteína asociada a la enfermedad de la necrosis hepatopancreatica aguda (AHPND) en Litopenaeus vannamei bajo cultivo semi-intensivo en Ecuador |
| title |
Detection of a protein associated with acute hepatopancreatic necrosis disease (AHPND) in Litopenaeus vannamei under semi-intensive farming in Ecuador |
| spellingShingle |
Detection of a protein associated with acute hepatopancreatic necrosis disease (AHPND) in Litopenaeus vannamei under semi-intensive farming in Ecuador Saavedra-Olivos, Katherine Yuliana shrimp AHPND Litopenaus vannamei early mortality syndrome Vibrio parahaemolitycus PirvpB camarón AHPND Litopenaeus vannamei síndrome de mortalidad precoz Vibrio parahaemolyticus PirvpB |
| title_short |
Detection of a protein associated with acute hepatopancreatic necrosis disease (AHPND) in Litopenaeus vannamei under semi-intensive farming in Ecuador |
| title_full |
Detection of a protein associated with acute hepatopancreatic necrosis disease (AHPND) in Litopenaeus vannamei under semi-intensive farming in Ecuador |
| title_fullStr |
Detection of a protein associated with acute hepatopancreatic necrosis disease (AHPND) in Litopenaeus vannamei under semi-intensive farming in Ecuador |
| title_full_unstemmed |
Detection of a protein associated with acute hepatopancreatic necrosis disease (AHPND) in Litopenaeus vannamei under semi-intensive farming in Ecuador |
| title_sort |
Detection of a protein associated with acute hepatopancreatic necrosis disease (AHPND) in Litopenaeus vannamei under semi-intensive farming in Ecuador |
| dc.creator.none.fl_str_mv |
Saavedra-Olivos, Katherine Yuliana Peralta-Ortiz, Tessy Ordinola-Zapata, Alberto Sandoval-Ramayoni, John Estuardo Vieyra-Peña, Enedia Graciela Zapata-Cruz, Marco Antonio Hidalgo-Mogollón, Auberto Morán, Braulio Mendoza-Neyra, Oscar Augusto Mendoza-Dioses, Magno Ego Campoverde-Peña, Sigrid Yamile |
| author |
Saavedra-Olivos, Katherine Yuliana |
| author_facet |
Saavedra-Olivos, Katherine Yuliana Peralta-Ortiz, Tessy Ordinola-Zapata, Alberto Sandoval-Ramayoni, John Estuardo Vieyra-Peña, Enedia Graciela Zapata-Cruz, Marco Antonio Hidalgo-Mogollón, Auberto Morán, Braulio Mendoza-Neyra, Oscar Augusto Mendoza-Dioses, Magno Ego Campoverde-Peña, Sigrid Yamile |
| author_role |
author |
| author2 |
Peralta-Ortiz, Tessy Ordinola-Zapata, Alberto Sandoval-Ramayoni, John Estuardo Vieyra-Peña, Enedia Graciela Zapata-Cruz, Marco Antonio Hidalgo-Mogollón, Auberto Morán, Braulio Mendoza-Neyra, Oscar Augusto Mendoza-Dioses, Magno Ego Campoverde-Peña, Sigrid Yamile |
| author2_role |
author author author author author author author author author author |
| dc.subject.none.fl_str_mv |
shrimp AHPND Litopenaus vannamei early mortality syndrome Vibrio parahaemolitycus PirvpB camarón AHPND Litopenaeus vannamei síndrome de mortalidad precoz Vibrio parahaemolyticus PirvpB |
| topic |
shrimp AHPND Litopenaus vannamei early mortality syndrome Vibrio parahaemolitycus PirvpB camarón AHPND Litopenaeus vannamei síndrome de mortalidad precoz Vibrio parahaemolyticus PirvpB |
| description |
The present study aimed to detect a protein associated with acute hepatopancreatic necrosis (AHPND) by mass spectrometry, reared under semi-intensive farming in Ecuador. Sick shrimps from three farms were collected in the Bellavista area in the El Oro province. The hepatopancreas were macerated and cultured in TCBS medium and subcultured in TSA and LB broth. In the bacterial strains obtained, the proteins were extracted using a commercial kit and separated by SDS-PAGE gel migration. These were analyzed with a MALDI TOF/TOF mass spectrometer. The confirmation of the strains was performed by PCR using TUMSAT-Vp3 primers, which are specific for detecting AHPND. One of the strains had peptide sequences similar to that of the PirvpB protein causing AHPND, and was identified as belonging to Vibrio parahaemolyticus and carrying the gene coding for PirvpB. The results showed that it is possible to use MALDI TOF/TOF mass spectrometry in the detection of AHPND-associated proteins in shrimp culture. |
| publishDate |
2018 |
| dc.date.none.fl_str_mv |
2018-03-14 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/14194 10.15381/rivep.v29i1.14194 |
| url |
https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/14194 |
| identifier_str_mv |
10.15381/rivep.v29i1.14194 |
| dc.language.none.fl_str_mv |
spa |
| language |
spa |
| dc.relation.none.fl_str_mv |
https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/14194/12767 https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/14194/14007 |
| dc.rights.none.fl_str_mv |
https://creativecommons.org/licenses/by-nc-sa/4.0 info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/4.0 |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf text/html |
| dc.publisher.none.fl_str_mv |
Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria |
| publisher.none.fl_str_mv |
Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria |
| dc.source.none.fl_str_mv |
Revista de Investigaciones Veterinarias del Perú; Vol. 29 Núm. 1 (2018); 328-338 Revista de Investigaciones Veterinarias del Perú; Vol. 29 No. 1 (2018); 328-338 1682-3419 1609-9117 reponame:Revistas - Universidad Nacional Mayor de San Marcos instname:Universidad Nacional Mayor de San Marcos instacron:UNMSM |
| instname_str |
Universidad Nacional Mayor de San Marcos |
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UNMSM |
| institution |
UNMSM |
| reponame_str |
Revistas - Universidad Nacional Mayor de San Marcos |
| collection |
Revistas - Universidad Nacional Mayor de San Marcos |
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| repository.mail.fl_str_mv |
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1795238228722188288 |
| score |
13.955691 |
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La información contenida en este registro es de entera responsabilidad de la institución que gestiona el repositorio institucional donde esta contenido este documento o set de datos. El CONCYTEC no se hace responsable por los contenidos (publicaciones y/o datos) accesibles a través del Repositorio Nacional Digital de Ciencia, Tecnología e Innovación de Acceso Abierto (ALICIA).
La información contenida en este registro es de entera responsabilidad de la institución que gestiona el repositorio institucional donde esta contenido este documento o set de datos. El CONCYTEC no se hace responsable por los contenidos (publicaciones y/o datos) accesibles a través del Repositorio Nacional Digital de Ciencia, Tecnología e Innovación de Acceso Abierto (ALICIA).
