Hydroxyapatite synthesized from the eggshell as a potential bone substitute in periodontal and peri-implant defects

• Autores: Camacho Díaz, Isabel, Vela Rodríguez, Melissa, Villanueva Aburto, Ludolfo, Borja Villanueva, Andrés, Montalvo Amanca, Fred, Quispe Marcatoma, Justiniano
• Formato: artículo
• Fecha de Publicación: 2018
• Institución: Universidad Nacional Mayor de San Marcos
• Repositorio: Revistas - Universidad Nacional Mayor de San Marcos
• Lenguaje: español
• OAI Identifier: oai:ojs.csi.unmsm:article/15557
• Enlace del recurso: https://revistasinvestigacion.unmsm.edu.pe/index.php/odont/article/view/15557
• Nivel de acceso: acceso abierto
• Materia: Cáscara de huevo; Difracción de rayos X; Hidroxiapatita; Precipitación química; Regeneración ósea; Bone regeneration; Chemical precipitation; Egg shell; Hydroxyapatite; X-Ray diffraction

Synthetic hydroxyapatite is a biomaterial widely used in guided bone regeneration procedures. Objective: Synthesize synthetic hydroxyapatite from eggshells under different precipitates of nitric acid. Methods: Experimental study, which obtained CaCO3 from the pulverization of eggshells (Gallus gallus). The carbonate was titrated with three concentrations of nitric acid (5, 10 and 15%) and ammonium phosphate. The mixtures were tested at different calcination temperatures (150, 600, 700, 800 and 900 °C) and calcination times (2, 4, 8 and 12 h). X-ray diffraction analysis was used to characterize the presence of calcium carbonate and hydroxyapatite. Results: Of the different routes of synthesis, the route of precipitation of nitric acid by excess to 15% plus calcined calcium carbonate at 700 °C during 8h offered more equivalent results to the synthetic hydroxyapatite when compared with the commercial standard. The peaks of intensity corresponded to the angles 2θ: 20, 26, 33, 34, 40, 48 and 50. Conclusions: The method of synthesis by precipitation, using calcium carbonate from eggshells as substrate plus calcium nitrate and nitric acid; proved to be adequate and practical for the synthesis of hydroxyapatite. Its use can be replicated for toxicological characterization and response studies in pre-clinical experimental models.