Objectives: To evaluate the performance of the enzyme-linked immunoelectrotransfer blot assay (EITB, Western blot) using excretory/secretory antigens from adult forms of Fasciola hepatica (Fh E/S Ag) for the diagnosis of human fasciolosis. Materials and methods: Antigens were obtained after 18 hours of incubation in culture medium Minimum Essential Eagle, prepared at a protein concentration of 0.15 ug/uL and run against a pool of sera of patients with proven fasciolosis (confirmed by the finding of parasite eggs in the stool microscopy). Antigens of 10, 12, 17, 23, 27, 30, 36, 43, 66 and 136 kDa were detected and used to develop the Western blot technique. The sensitivity was evaluated using sera from 67 fasciolosis patients, and the specificity using sera from 57 patients with other parasitic diseases, and 10 from healthy individuals. Results: Out of the 67 sera, 64 reacted with the 23 ...

In recent years, the use of chatbots in higher education has grown significantly, driven by advances in artificial intelligence and its ability to optimize teaching and learning processes. This paper presents a systematic literature review aimed at identifying the main trends, approaches, and findings related to the use of chatbots in higher education. An exhaustive search was conducted across five highly reputable academic databases (Scopus, EBSCOhost, ScienceDirect, Taylor & Francis Online, and ARDI), yielding an initial total of 42,188 documents. After applying strict inclusion and exclusion criteria, 69 scientific papers were selected for detailed analysis. The findings reveal that the most widely used programming language in the development of educational chatbots is R (45.2%), followed by Python and Java (22.5% each). In addition, 66.6% of the analyzed papers were published in Q1 j...